Identification and Characterization of Citrus Peel Uronic Acid Oxidase

Yifeng Wei, Yee Ling Tan, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

Uronic acid-rich plant materials such as pectin are potential renewable feedstocks for the chemical industry. Uronic acid oxidase activity was first reported in extracts of citrus leaves, and was subsequently found to be widely distributed in plants, with the highest activity detected in extracts of the pectin-rich citrus peel. Herein we report the identification of the primary sequence of uronic acid oxidase from extracts of the peel of Citrus sinensis, by partial purification and protein mass spectrometry. Activity of the enzyme, a member of the berberine bridge family, was confirmed by recombinant expression in Pichia pastoris. Biochemical characterization of the recombinant enzyme is reported. Our findings facilitate further investigation of the biological function of uronic acid oxidation in the economically important orange fruit, and also provide a basis for the development of a catalyst for bioconversion of uronic acids.

Original languageEnglish (US)
Pages (from-to)797-800
Number of pages4
JournalChemBioChem
Volume21
Issue number6
DOIs
StatePublished - Mar 16 2020

Keywords

  • Citrus sinensis
  • berberine bridge enzyme
  • enzyme catalysis
  • green chemistry
  • uronic acid oxidase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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