TY - JOUR
T1 - Identification and characterization of andalusicin
T2 - N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis
AU - Grigoreva, Anastasiia
AU - Andreeva, Julia
AU - Bikmetov, Dmitry
AU - Rusanova, Anastasiia
AU - Serebryakova, Marina
AU - Garcia, Andrea Hernandez
AU - Slonova, Darya
AU - Nair, Satish K.
AU - Lippens, Guy
AU - Severinov, Konstantin
AU - Dubiley, Svetlana
N1 - We are grateful to Dmitry Travin (Skoltech, Russia) and Dmitry Ghilarov (Jagiellonian University, Poland) for the critical reading of the manuscript. We thank Tatyana Smirnova and the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, IGB RAS (Moscow, Russia), for microscopy. We thank Dr. Viktor Zgoda and “Human Proteome” Core Facilities of the Institute of Biomedical Chemistry (Moscow, Russia) for high-resolution mass spectrometry. We are grateful to Prof. V. Minaev and the Shared Use Equipment Center for high-precision measuring in photonics (ckp.vniiofi.ru, VNIIOFI, Moscow, Russia) for help with optical rotation measurement. This work was supported by the Russian Foundation of Basic Research grant RFBR 19-04-01163 to J.A., NIH RO1 grant AI117270 (S.K.N. and K.S.), and GM079038 (S.K.N.). Mutational analysis of andalusicin was supported by Russian Science Foundation grant RSF 19-14-00266 to S.D. MALDI-TOF-MS facility became available to us in the framework of the Moscow State University Development Program PNG 5.13. The NMR facility is funded through MetaToul (Toulouse metabolomics & fluxomics facilities, www.metatoul.fr ) as part of the French National Infrastructure for Metabolomics and Fluxomics MetaboHUB-AR-11-INBS-0010 ( www.metabohub.fr ). It receives support from the Région Midi-Pyrénées, the ERDF, the SICOVAL, and the French Minister of Education & Research, who are all gratefully acknowledged.
We are grateful to Dmitry Travin (Skoltech, Russia) and Dmitry Ghilarov (Jagiellonian University, Poland) for the critical reading of the manuscript. We thank Tatyana Smirnova and the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, IGB RAS (Moscow, Russia), for microscopy. We thank Dr. Viktor Zgoda and ?Human Proteome? Core Facilities of the Institute of Biomedical Chemistry (Moscow, Russia) for high-resolution mass spectrometry. We are grateful to Prof. V. Minaev and the Shared Use Equipment Center for high-precision measuring in photonics (ckp.vniiofi.ru, VNIIOFI, Moscow, Russia) for help with optical rotation measurement. This work was supported by the Russian Foundation of Basic Research grant RFBR 19-04-01163 to J.A. NIH RO1 grant AI117270 (S.K.N. and K.S.), and GM079038 (S.K.N.). Mutational analysis of andalusicin was supported by Russian Science Foundation grant RSF 19-14-00266 to S.D. MALDI-TOF-MS facility became available to us in the framework of the Moscow State University Development Program PNG 5.13. The NMR facility is funded through MetaToul (Toulouse metabolomics & fluxomics facilities, www.metatoul.fr) as part of the French National Infrastructure for Metabolomics and Fluxomics MetaboHUB-AR-11-INBS-0010 (www.metabohub.fr). It receives support from the R?gion Midi-Pyr?n?es, the ERDF, the SICOVAL, and the French Minister of Education & Research, who are all gratefully acknowledged. Conceptualization, S.D.; methodology, S.K.N. M.S. and S.D.; investigation, A.G. A.R. J.A. D.B. M.S. D.S. A.H.G. and G.L.; writing ? original draft, S.D.; writing ? review & editing, S.K.N. and K.S.; resources, M.S. and G.L.; supervision, K.S. and S.D. The authors declare no competing interests.
PY - 2021/5/21
Y1 - 2021/5/21
N2 - Lanthipeptides, ribosomally synthesized and post-translationally modified peptides (RiPPs), can be divided into five classes based on their structures and biosynthetic pathways. Class I and II lanthipeptides have been well characterized, whereas less is known about members of the other three classes. Here, we describe a new family of class III lanthipeptides from Firmicutes. Members of the family are distinguished by the presence of a single carboxy-terminal labionin. We identified and characterized andalusicin, a representative of this family. Andalusicin bears two methyl groups at the α-amino terminus, a post-translational modification that has not previously been identified in class III lanthipeptides. Mature andalusicin A shows bioactivity against various Gram-positive bacteria, an activity that is highly dependent on the α-N dimethylation.
AB - Lanthipeptides, ribosomally synthesized and post-translationally modified peptides (RiPPs), can be divided into five classes based on their structures and biosynthetic pathways. Class I and II lanthipeptides have been well characterized, whereas less is known about members of the other three classes. Here, we describe a new family of class III lanthipeptides from Firmicutes. Members of the family are distinguished by the presence of a single carboxy-terminal labionin. We identified and characterized andalusicin, a representative of this family. Andalusicin bears two methyl groups at the α-amino terminus, a post-translational modification that has not previously been identified in class III lanthipeptides. Mature andalusicin A shows bioactivity against various Gram-positive bacteria, an activity that is highly dependent on the α-N dimethylation.
KW - Biochemistry
KW - Biosynthesis
KW - Chemistry
KW - Medical biochemistry
UR - http://www.scopus.com/inward/record.url?scp=85105543930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85105543930&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2021.102480
DO - 10.1016/j.isci.2021.102480
M3 - Article
C2 - 34113822
AN - SCOPUS:85105543930
SN - 2589-0042
VL - 24
JO - iScience
JF - iScience
IS - 5
M1 - 102480
ER -