TY - JOUR
T1 - Identification and Analysis of the Putative Pentose Sugar Efflux Transporters in Escherichia coli
AU - Koita, Khushnuma
AU - Rao, Christopher V.
PY - 2012/8/28
Y1 - 2012/8/28
N2 - Escherichia coli possesses a number of proteins that transport sugars out of the cell. We identified 31 candidate sugar efflux transporters based on their similarity to known sugar efflux transporters. We then tested whether these transporters affect arabinose and xylose metabolism. We identified 13 transporters - setC, cmr, ynfM, mdtD, yfcJ, yhhS, emrD, ydhC, ydeA, ybdA, ydeE, mhpT, and kgtP - that appeared to increase or decrease intracellular arabinose concentrations when respectively deleted or over-expressed. None of the candidate transporters affected xylose concentrations. These results indicate that E. coli possesses multiple arabinose efflux transporters. They also provide a novel target for future metabolic engineering.
AB - Escherichia coli possesses a number of proteins that transport sugars out of the cell. We identified 31 candidate sugar efflux transporters based on their similarity to known sugar efflux transporters. We then tested whether these transporters affect arabinose and xylose metabolism. We identified 13 transporters - setC, cmr, ynfM, mdtD, yfcJ, yhhS, emrD, ydhC, ydeA, ybdA, ydeE, mhpT, and kgtP - that appeared to increase or decrease intracellular arabinose concentrations when respectively deleted or over-expressed. None of the candidate transporters affected xylose concentrations. These results indicate that E. coli possesses multiple arabinose efflux transporters. They also provide a novel target for future metabolic engineering.
UR - http://www.scopus.com/inward/record.url?scp=84865512261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865512261&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0043700
DO - 10.1371/journal.pone.0043700
M3 - Article
C2 - 22952739
AN - SCOPUS:84865512261
SN - 1932-6203
VL - 7
JO - PloS one
JF - PloS one
IS - 8
M1 - e43700
ER -