TY - JOUR
T1 - ICAM-1 expression in autoimmune encephalitis visualized using magnetic resonance imaging
AU - Sipkins, Dorothy A.
AU - Gijbels, Koenraad
AU - Tropper, François D.
AU - Bednarski, Mark
AU - Li, King C.P.
AU - Steinman, Lawrence
N1 - Supported by a Sterling-Winthrop Chimera grant and by a grant from the National Institute of General Medical Sciences 5T32 GM07365 to D.A.S. The high resolution MRI images were obtained at the Center for In Vivo Microscopy, Department of Radiology, Duke University Medical Center, Durham, NC, under the auspices of Professor G. Allan Johnson and with the assistance of Gary P. Cofer and Bradley R. Smith. We thank Henry Li, H. William Strauss, Raymond Sobel, Eugene Butcher, and Ellen Berg for helpful discussions, and Bayard Colyear for help in preparation of the figures.
PY - 2000/4/3
Y1 - 2000/4/3
N2 - The expression of leukocyte adhesion molecules in the intact brains of mice with experimental autoimmune encephalitis (EAE) was visualized by Magnetic Resonance Imaging (MRI) through the use of a new, target-specific MR contrast agent. Antibody-conjugated paramagnetic liposomes (ACPLs) were designed to achieve in vivo targeting of molecules expressed on vascular endothelium, while providing sufficient signal enhancement at these sites for detection by MRI. ACPLs targeted to intercellular adhesion molecule-1 (ICAM-1), an endothelial leukocyte receptor upregulated on cerebral microvasculature during EAE, were administered to diseased mice. Fluorescence microscopy confirmed that fluorescently-tagged ACPLs were localized to central nervous system (CNS) microvasculature in a pattern consistent with ICAM-1 upregulation described immunohistochemically. High resolution MRI of mouse brains ex vivo demonstrated that ACPL binding conferred significant enhancement of signal intensity (SI) as compared to control images. These results suggest that ACPLs can be used as MRI contrast agents to visualize specific molecules expressed on vascular endothelium during disease. (C) 2000 Elsevier Science B.V.
AB - The expression of leukocyte adhesion molecules in the intact brains of mice with experimental autoimmune encephalitis (EAE) was visualized by Magnetic Resonance Imaging (MRI) through the use of a new, target-specific MR contrast agent. Antibody-conjugated paramagnetic liposomes (ACPLs) were designed to achieve in vivo targeting of molecules expressed on vascular endothelium, while providing sufficient signal enhancement at these sites for detection by MRI. ACPLs targeted to intercellular adhesion molecule-1 (ICAM-1), an endothelial leukocyte receptor upregulated on cerebral microvasculature during EAE, were administered to diseased mice. Fluorescence microscopy confirmed that fluorescently-tagged ACPLs were localized to central nervous system (CNS) microvasculature in a pattern consistent with ICAM-1 upregulation described immunohistochemically. High resolution MRI of mouse brains ex vivo demonstrated that ACPL binding conferred significant enhancement of signal intensity (SI) as compared to control images. These results suggest that ACPLs can be used as MRI contrast agents to visualize specific molecules expressed on vascular endothelium during disease. (C) 2000 Elsevier Science B.V.
KW - Intracellular adhesion molecules (ICAM)
KW - Magnetic resonance imaging (MRI)
KW - Multiple sclerosis (MS)
UR - https://www.scopus.com/pages/publications/0033952596
UR - https://www.scopus.com/pages/publications/0033952596#tab=citedBy
U2 - 10.1016/S0165-5728(99)00248-9
DO - 10.1016/S0165-5728(99)00248-9
M3 - Article
C2 - 10683508
AN - SCOPUS:0033952596
SN - 0165-5728
VL - 104
SP - 1
EP - 9
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -