TY - JOUR
T1 - IC-tagged proteins are able to interact with each other and perform complex reactions when integrated into muNS-derived inclusions
AU - Brandariz-Nuñez, Alberto
AU - Otero-Romero, Iria
AU - Benavente, Javier
AU - Martinez-Costas, Jose M.
N1 - This work was supported by grants from the European Commission under contracts ERAS-CT-2003-980409 (as part of the European Science Foundation EUROCORES Programme EuroSCOPE, web – http://www.esf.org/euroscope ); the Spanish Ministerio de Ciencia y Tecnología ( BFU2007-61330 , BFU 205-24982-E , web: http://www.mityc.es/ ) and Xunta de Galicia ( 08CSA009203PR , web – http://www.conselleriaiei.org/ga/dxidi/index.php ). ABN and IOR were recipients of a predoctoral FPI fellowship from the Spanish Ministerio de Ciencia y Tecnología. We thank Rebeca Menaya Vargas and Leticia Barcia Castro for excellent technical assistance.
PY - 2011/9/20
Y1 - 2011/9/20
N2 - We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.
AB - We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.
KW - IC-tagging
KW - Particulate protein inclusions
KW - Protein-protein interactions
KW - Reovirus
UR - https://www.scopus.com/pages/publications/80052378147
UR - https://www.scopus.com/pages/publications/80052378147#tab=citedBy
U2 - 10.1016/j.jbiotec.2011.07.010
DO - 10.1016/j.jbiotec.2011.07.010
M3 - Article
C2 - 21802457
AN - SCOPUS:80052378147
SN - 0168-1656
VL - 155
SP - 284
EP - 286
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 3
ER -