Hypervirulent Klebsiella pneumoniae in California Sea Lions (Zalophus californianus): Pathologic Findings in Natural Infections

Mauricio Seguel, Nicole L. Gottdenker, Kathleen Colegrove, Shawn Johnson, Carsten Struve, Elizabeth W. Howerth

Research output: Contribution to journalArticlepeer-review

Abstract

Tissues of stranded California sea lions (Zalophus californianus) naturally infected with a hyperviruluent strain of Klebsiella pneumoniae were examined by histopathology and immunohistochemistry against the K. pneumoniae K2 capsular antigen. In 7 of 8 animals, there was severe purulent bronchopneumonia, sometimes complicated by fibrinonecrotizing pleuritis with pyothorax. In affected areas of lung, large numbers of degenerate neutrophils and macrophages were admixed with rare large extracellular and intracellular gram-negative bacilli surrounded by a prominent capsule. Through serotyping, polymerase chain reaction, sequencing, and immunohistochemistry, these bacteria were confirmed to be a K2 serotype of K. pneumoniae. The same bacteria were identified through double immunolabeling within macrophages in blood vessels, lymph nodes, spleen, and liver. Intact K. pneumoniae were identified on epithelial surfaces of the nasopharyngeal, tracheal, and small intestine mucosae and within distal renal tubules. Our findings indicate that hypervirulent K. pneumoniae causes severe respiratory disease and intrahistiocytic bacteremia in California sea lions.

Original languageEnglish (US)
Pages (from-to)846-850
Number of pages5
JournalVeterinary pathology
Volume54
Issue number5
DOIs
StatePublished - Sep 1 2017

Keywords

  • California sea lions
  • Klebsiella pneumoniae K2 serotype
  • Zalophus californianus
  • bacteremia
  • hypermucoviscous
  • hypervirulent
  • immunohistochemistry

ASJC Scopus subject areas

  • veterinary(all)

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