Hypertonicity activates GSK3β in tumor cells

Pablo Perez-Pinera, Manuel Menendez-Gonzalez, Miguel del Valle, Jose Antonio Vega

Research output: Contribution to journalArticlepeer-review


Responses to perturbations in the composition of the extracellular environment are crucial to maintain cell and tissue homeostasis. In hypertonic conditions cell lines derived from kidney epithelium initiate a variety of stress responses to maintain cell viability that include activation of Mitogen Activated Protein Kinases (MAPK). We previously showed that NaCl also regulates MAPK in different tumor cell lines and we now show that when hypertonic conditions induced with NaCl and other osmolytes were used to stimulate several tumor cell lines, Glycogen Synthase Kinase 3β (GSK3β) was rapidly dephosphorylated at serine 9 and its kinase activity was increased. This response was both time- and dose-dependent, it was independent of the Akt signaling pathway and did not increase steady state levels of phosphorylation of β-catenin, although the data suggested that activated GSK3β could regulate the activity of ERK1/2.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalMolecular and Cellular Biochemistry
Issue number1-2
StatePublished - Oct 2006
Externally publishedYes


  • Cell signaling
  • GSK3β
  • Hypertonicity
  • NaCl

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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