The anion radicals of ubiquinones-10 13C chemically labeled at the C5 or C6 ring positions in alcohol have been studied by 1D and 2D ESEEM to define the hyperfine interaction tensors with the 13C nuclei. Analysis of the cross-peak line shapes and simulations of the spectra allowed us to conclude that the hyperfine tensors are characterized by an anisotropic component T ~6 MHz and an isotropic coupling a ~-3 MHz with support from DFT calculations. However, these values were found to be inconsistent with the shift of the sum combination harmonic in the four-pulse ESEEM spectra. Simulations resolve this apparent discrepancy by showing that the shift of the sum combination to lower frequency and its broadening can be accounted for by a distribution of the hyperfine couplings. A spread of the methoxy group conformations, as supported by previous experimental observations, is suggested as the mechanism influencing the distribution of the hyperfine couplings for the ring carbons.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics