Hyperactive mutants of Himar1 transposase and methods for using the same

David J. Lampe (Inventor), Hugh Robertson (Inventor), Eric Rubin (Inventor), Brian Akerley (Inventor)

Research output: Patent


Mariner-family transposable elements are active in a wide variety of organisms and are becoming increasingly important genetic tools in species lacking sophisticated genetics. The Himar1 element, a member of the mariner family, isolated from the horn fly, Haematobia irritans, is active in Escherichia coli when expressed appropriately. Using this fact, a genetic screen was devised to isolate hyperactive mutants of Himar1 transposase that enhance overall transposition from 4 to 50-fold as measured in an E. coli assay. These hyperactive Himar1 mutant transposases should enable sophisticated analysis of the biochemistry of mariner transposition and should improve efficiency of a variety of genetic manipulations involving transposition in vivo and in vitro such as random mutagenesis or transgenesis in a wide range of host cells than the transposable elements previously available.
Original languageEnglish (US)
U.S. patent number6368830
Filing date9/27/00
StatePublished - Apr 9 2002


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