Hydrothermal cell disruption of Nannochloropsis sp. and its influence on lipid extraction

Zhi Qu; Jianli Zeng; Yuanhui Zhang; Qiang Liao; Brajendra Kumar Sharma; Qian Fu; Yun Huang; Zhidan Liu

doi:10.1016/j.algal.2018.09.015

Hydrothermal cell disruption of Nannochloropsis sp. and its influence on lipid extraction

Zhi Qu, Jianli Zeng, Yuanhui Zhang, Qiang Liao, Brajendra Kumar Sharma, Qian Fu, Yun Huang, Zhidan Liu

Research output: Contribution to journal › Article › peer-review

Abstract

Cell wall disruption is a critical challenge for algal biofuel. Here, we reported Hydrothermal treatment (HTT) of high solid content (20% w/w) Nannochloropsis sp. and its influence on extraction of lipid. Various process variables were specifically studied including reaction temperature (120–200 °C) and retention time (0–60 min). SEM and TEM images revealed the cell morphology before and after HTT. A high extraction yield (84%) of lipid was obtained at 180 °C with a retention time of 60 min, without the use of any catalysts. GC–MS revealed that the main compounds in the extracts were fatty acids, hydrocarbons and esters. UPLC-MS/MS uncovered the detailed varieties of glyceride, phospholipid and glycolipid, and demonstrated that (16:0/16:0/16:1) triglyceride (TAG) was the most abundant TAG. HTT might be a promising method to break microalgae cell wall for lipid extraction.

Original language	English (US)
Pages (from-to)	407-415
Number of pages	9
Journal	Algal Research
Volume	35
DOIs	https://doi.org/10.1016/j.algal.2018.09.015
State	Published - Nov 2018

Keywords

Algae
Algal biofuel
Cell wall disruption
Hydrothermal treatment
Lipid extraction

ASJC Scopus subject areas

Agronomy and Crop Science

Online availability

10.1016/j.algal.2018.09.015

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title = "Hydrothermal cell disruption of Nannochloropsis sp. and its influence on lipid extraction",

abstract = "Cell wall disruption is a critical challenge for algal biofuel. Here, we reported Hydrothermal treatment (HTT) of high solid content (20% w/w) Nannochloropsis sp. and its influence on extraction of lipid. Various process variables were specifically studied including reaction temperature (120–200 °C) and retention time (0–60 min). SEM and TEM images revealed the cell morphology before and after HTT. A high extraction yield (84%) of lipid was obtained at 180 °C with a retention time of 60 min, without the use of any catalysts. GC–MS revealed that the main compounds in the extracts were fatty acids, hydrocarbons and esters. UPLC-MS/MS uncovered the detailed varieties of glyceride, phospholipid and glycolipid, and demonstrated that (16:0/16:0/16:1) triglyceride (TAG) was the most abundant TAG. HTT might be a promising method to break microalgae cell wall for lipid extraction.",

keywords = "Algae, Algal biofuel, Cell wall disruption, Hydrothermal treatment, Lipid extraction",

author = "Zhi Qu and Jianli Zeng and Yuanhui Zhang and Qiang Liao and Sharma, {Brajendra Kumar} and Qian Fu and Yun Huang and Zhidan Liu",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = nov,

doi = "10.1016/j.algal.2018.09.015",

language = "English (US)",

volume = "35",

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journal = "Algal Research",

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T1 - Hydrothermal cell disruption of Nannochloropsis sp. and its influence on lipid extraction

AU - Qu, Zhi

AU - Zeng, Jianli

AU - Zhang, Yuanhui

AU - Liao, Qiang

AU - Sharma, Brajendra Kumar

AU - Fu, Qian

AU - Huang, Yun

AU - Liu, Zhidan

PY - 2018/11

Y1 - 2018/11

N2 - Cell wall disruption is a critical challenge for algal biofuel. Here, we reported Hydrothermal treatment (HTT) of high solid content (20% w/w) Nannochloropsis sp. and its influence on extraction of lipid. Various process variables were specifically studied including reaction temperature (120–200 °C) and retention time (0–60 min). SEM and TEM images revealed the cell morphology before and after HTT. A high extraction yield (84%) of lipid was obtained at 180 °C with a retention time of 60 min, without the use of any catalysts. GC–MS revealed that the main compounds in the extracts were fatty acids, hydrocarbons and esters. UPLC-MS/MS uncovered the detailed varieties of glyceride, phospholipid and glycolipid, and demonstrated that (16:0/16:0/16:1) triglyceride (TAG) was the most abundant TAG. HTT might be a promising method to break microalgae cell wall for lipid extraction.

AB - Cell wall disruption is a critical challenge for algal biofuel. Here, we reported Hydrothermal treatment (HTT) of high solid content (20% w/w) Nannochloropsis sp. and its influence on extraction of lipid. Various process variables were specifically studied including reaction temperature (120–200 °C) and retention time (0–60 min). SEM and TEM images revealed the cell morphology before and after HTT. A high extraction yield (84%) of lipid was obtained at 180 °C with a retention time of 60 min, without the use of any catalysts. GC–MS revealed that the main compounds in the extracts were fatty acids, hydrocarbons and esters. UPLC-MS/MS uncovered the detailed varieties of glyceride, phospholipid and glycolipid, and demonstrated that (16:0/16:0/16:1) triglyceride (TAG) was the most abundant TAG. HTT might be a promising method to break microalgae cell wall for lipid extraction.

KW - Algae

KW - Algal biofuel

KW - Cell wall disruption

KW - Hydrothermal treatment

KW - Lipid extraction

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DO - 10.1016/j.algal.2018.09.015

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SN - 2211-9264

VL - 35

SP - 407

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JO - Algal Research

JF - Algal Research

ER -

Hydrothermal cell disruption of Nannochloropsis sp. and its influence on lipid extraction

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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