TY - GEN
T1 - Hydrodynamic stretching of single cells for high-throughput vector-free intracellular delivery of macromolecules
AU - Deng, Yanxiang
AU - Kizer, Megan
AU - Wang, Xing
AU - Chung, Aram J.
N1 - Funding Information:
A.J.C. acknowledges funding from RPI, KSEA Young Investigator Grant (H71175/2280), and National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A1B07045538). X.W. acknowledges funding from RPI, CBIS at RPI, and the gift funds from HT Materials Corporation. The authors thank Dr. Sergey Pryshchep at RPI, and Ms. Erin Maloney at University at Buffalo for their technical support and useful comments.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - We present a novel high-throughput vector-free macromolecule intracellular delivery microfluidic platform. The platform inertially focuses cells into the channel center and then guides them to be uniformly stretched via extensional flow near stagnation point. Hydrodynamic cell deformation creates nanopores on cellular and nuclear membranes that facilitate the transport of exogenous materials into the cell cytoplasm. The presented approach can effectively deliver a wide range of nanoscopic cargos to different cell types with high efficiency while maintaining high cell viability. This hydroporation method through cell stretching shows superior delivery efficiency, is high-throughput, simple, low-cost and clogging-free, and has high controllability.
AB - We present a novel high-throughput vector-free macromolecule intracellular delivery microfluidic platform. The platform inertially focuses cells into the channel center and then guides them to be uniformly stretched via extensional flow near stagnation point. Hydrodynamic cell deformation creates nanopores on cellular and nuclear membranes that facilitate the transport of exogenous materials into the cell cytoplasm. The presented approach can effectively deliver a wide range of nanoscopic cargos to different cell types with high efficiency while maintaining high cell viability. This hydroporation method through cell stretching shows superior delivery efficiency, is high-throughput, simple, low-cost and clogging-free, and has high controllability.
KW - Hydroporation
KW - Inertial microfluidics
KW - Intracellular delivery
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M3 - Conference contribution
AN - SCOPUS:85079736311
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1352
EP - 1354
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -