Human dipeptidyl peptidase III regulates G-protein coupled receptor-dependent Ca2+ concentration in human embryonic kidney 293T cells

Subhash C. Prajapati; Ratnakar Singh; Shyam S. Chauhan

doi:10.1515/hsz-2016-0117

Human dipeptidyl peptidase III regulates G-protein coupled receptor-dependent Ca²⁺ concentration in human embryonic kidney 293T cells

Subhash C. Prajapati, Ratnakar Singh, Shyam S. Chauhan

Research output: Contribution to journal › Article › peer-review

Abstract

The precise biological function of human dipeptidyl peptidase III (hDPP III) is poorly understood. Using luciferase reporter constructs responsive to change in Ca²⁺ and/or cAMP and Fura 2-AM fluorometric assay, we show a significant decrease in intracellular Ca²⁺ following hDPP III overexpression and angiotensin II stimulation in angiotensin II type 1 receptor (G-protein coupled receptor, GPCR) expressing HEK293T cells. Silencing the expression of hDPP III by siRNA reversed the effect of hDPP III overexpression with a concomitant increase in Ca²⁺. These results, for the first time, show involvement of hDPP III in GPCR dependent Ca²⁺ regulation in HEK293T cells.

Original language	English (US)
Pages (from-to)	563-569
Number of pages	7
Journal	Biological Chemistry
Volume	397
Issue number	6
DOIs	https://doi.org/10.1515/hsz-2016-0117
State	Published - Jun 1 2016
Externally published	Yes

Keywords

angiotensin II type I receptor
cAMP
cytosolic peptidase
intracellular peptide
M49 family
protein turnover

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Clinical Biochemistry

Online availability

10.1515/hsz-2016-0117

Library availability

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Cite this

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title = "Human dipeptidyl peptidase III regulates G-protein coupled receptor-dependent Ca2+ concentration in human embryonic kidney 293T cells",

abstract = "The precise biological function of human dipeptidyl peptidase III (hDPP III) is poorly understood. Using luciferase reporter constructs responsive to change in Ca2+ and/or cAMP and Fura 2-AM fluorometric assay, we show a significant decrease in intracellular Ca2+ following hDPP III overexpression and angiotensin II stimulation in angiotensin II type 1 receptor (G-protein coupled receptor, GPCR) expressing HEK293T cells. Silencing the expression of hDPP III by siRNA reversed the effect of hDPP III overexpression with a concomitant increase in Ca2+. These results, for the first time, show involvement of hDPP III in GPCR dependent Ca2+ regulation in HEK293T cells.",

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