Human Cytochrome CYP17A1: The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone

Piotr J. Mak, Ruchia Duggal, Ilia G. Denisov, Michael C. Gregory, Stephen Sligar, James R. Kincaid

Research output: Contribution to journalArticle

Abstract

The multifunctional enzyme, cytochrome P450 (CYP17A1), plays a crucial role in the production of androgens, catalyzing two key reactions on pregnenolone (PREG) and progesterone (PROG), the first being a 17-hydroxylation to generate 17-OH PREG and 17-OH PROG, with roughly equal efficiencies. The second is a C-C bond scission or "lyase" reaction in which the C17-C20 bond is cleaved, leading to the eventual production of powerful androgens, whose involvement in the proliferation of prostate cancer has generated intense interest in developing inhibitors of CYP17A1. For humans, the significance of the C-C bond cleavage of 17-OH PROG is lessened, because it is about 50 times less efficient than for 17-OH PREG in terms of kcat/Km. Recognizing the need to clarify relevant reaction mechanisms involved with such transformations, we first report studies of solvent isotope effects, results of which are consistent with a Compound I mediated PROG hydroxylase activity, yet exclude this intermediate as a participant in the formation of androstenedione (AD) via the lyase reaction. This finding is also supported by a combination of cryoreduction and resonance Raman spectroscopy that traps and structurally characterizes the key hemiketal reaction intermediates. Adding to a previous study of PREG and 17-OH PREG metabolism, the current work provides definitive evidence for a more facile protonation of the initially formed ferric peroxo-intermediate for 17-OH PROG-bound CYP17A1, compared to the complex with 17-OH PREG. Importantly, Raman characterization also reveals an H-bonding interaction with the terminal oxygen of the peroxo fragment, rather than with the proximal oxygen, as is present for 17-OH PREG. These factors would favor a diminished lyase activity of the sample with 17-OH PROG relative to the complex with 17-OH PREG, thereby providing a convincing structural explanation for the dramatic differences in activity for these lyase substrates in humans.

Original languageEnglish (US)
Pages (from-to)7324-7331
Number of pages8
JournalJournal of the American Chemical Society
Volume140
Issue number23
DOIs
StatePublished - Jun 13 2018

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17-alpha-Hydroxyprogesterone
Pregnenolone
Lyases
androgen
Cytochromes
cytochrome
Proteins
Progesterone
Reaction intermediates
Hydroxylation
oxygen
Oxygen
Protonation
Raman spectroscopy
Metabolism
cleavage
Isotopes
inhibitor
cancer
Enzymes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Human Cytochrome CYP17A1 : The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone. / Mak, Piotr J.; Duggal, Ruchia; Denisov, Ilia G.; Gregory, Michael C.; Sligar, Stephen; Kincaid, James R.

In: Journal of the American Chemical Society, Vol. 140, No. 23, 13.06.2018, p. 7324-7331.

Research output: Contribution to journalArticle

Mak, Piotr J. ; Duggal, Ruchia ; Denisov, Ilia G. ; Gregory, Michael C. ; Sligar, Stephen ; Kincaid, James R. / Human Cytochrome CYP17A1 : The Structural Basis for Compromised Lyase Activity with 17-Hydroxyprogesterone. In: Journal of the American Chemical Society. 2018 ; Vol. 140, No. 23. pp. 7324-7331.
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