Homologous regulation of estrogen receptor subtypes in goldfish (Carassius auratus)

E. R. Nelson, W. B. Wiehler, W. C. Cole, H. R. Habibi

Research output: Contribution to journalArticle

Abstract

While considerable information is available on the physiological effects of estrogen, much less is known about the regulation of estrogen receptor (ER) subtypes, particularly in non-mammalian vertebrates. Using goldfish as primary experimental model, we investigated sex- and tissue-specific homologous regulation of ER subtypes (ERα, ERβI, and ERβII) by estradiol in vivo, in the liver and gonads. Treatment with estradiol, significantly upregulated transcript levels for all three types of ERs (ERα, ERβI, and ERβII) in the goldfish ovary and testis. In the goldfish liver, treatment with estradiol significantly increased ERα, ERβI transcript levels without affecting ERβII. In all cases increased ER transcript level was correlated with increased ER protein level determined by Western blot analysis, although we are not able to distinguish between ER subtypes. The results provide strong support for the hypothesis that homologous regulation of ERs is tissue- and gender-specific, and may be a mechanism for estrogen-mediated regulation of reproduction in goldfish.

Original languageEnglish (US)
Pages (from-to)1105-1112
Number of pages8
JournalMolecular reproduction and development
Volume74
Issue number9
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Goldfish
Estrogen Receptors
Estradiol
Estrogens
Liver
Gonads
Reproduction
Vertebrates
Testis
Ovary
Theoretical Models
Western Blotting

Keywords

  • Estrogen
  • Estrogen receptors
  • Goldfish
  • Homologous regulation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Homologous regulation of estrogen receptor subtypes in goldfish (Carassius auratus). / Nelson, E. R.; Wiehler, W. B.; Cole, W. C.; Habibi, H. R.

In: Molecular reproduction and development, Vol. 74, No. 9, 01.09.2007, p. 1105-1112.

Research output: Contribution to journalArticle

Nelson, E. R. ; Wiehler, W. B. ; Cole, W. C. ; Habibi, H. R. / Homologous regulation of estrogen receptor subtypes in goldfish (Carassius auratus). In: Molecular reproduction and development. 2007 ; Vol. 74, No. 9. pp. 1105-1112.
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