TY - JOUR
T1 - hnRNP G
T2 - Sequence and characterization of a glycosylated RNA-binding protein
AU - Soulard, Michel
AU - Valle, Veèronique Della
AU - Siomi, Mikkiko C.
AU - Pinol-roma, Serafin
AU - Codogno, Patrice
AU - Bauvy, Chantal
AU - Bellini, Michel
AU - Lacroix, Jean claude
AU - Monod, Guillaume
AU - Dreyfuss, Gidden
AU - Larsen, Christian jacques
N1 - Funding Information:
This work was supported by a Grant from the 'association pour la Recherche contre le Cancer de Villejuif (ARC) to CJL and by the Howard Hughes Medical Institute and grants from the NTH to GD.
PY - 1993/9/11
Y1 - 1993/9/11
N2 - The autoantlgen p43 is a nuclear protein initially identified with autoantibodles from dogs with a lupuslike syndrome. Here we show that p43 Is an RNAblnding protein, and identify it as hnRNP G, a previously described component of heterogeneous nuclear ribonucleoproteln complexes. We demonstrate that p43/hnRNP G Is glycosylated, and identify the modification as O-linked N-acetylglucosamine. A fulllength cDNA clone for hnRNP G has been isolated and sequenced, and the predicted amlno acid sequence for hnRNP G shows that It contains one RNP-consensus RNA binding domain (RBD) at the amlno terminus and a carboxyl domain rich In serines, arglnines and glyclnes. The RBD of human hnRNP G shows striking similarities with the RBDs of several plant RNA-blndlng proteins.
AB - The autoantlgen p43 is a nuclear protein initially identified with autoantibodles from dogs with a lupuslike syndrome. Here we show that p43 Is an RNAblnding protein, and identify it as hnRNP G, a previously described component of heterogeneous nuclear ribonucleoproteln complexes. We demonstrate that p43/hnRNP G Is glycosylated, and identify the modification as O-linked N-acetylglucosamine. A fulllength cDNA clone for hnRNP G has been isolated and sequenced, and the predicted amlno acid sequence for hnRNP G shows that It contains one RNP-consensus RNA binding domain (RBD) at the amlno terminus and a carboxyl domain rich In serines, arglnines and glyclnes. The RBD of human hnRNP G shows striking similarities with the RBDs of several plant RNA-blndlng proteins.
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U2 - 10.1093/nar/21.18.4210
DO - 10.1093/nar/21.18.4210
M3 - Article
C2 - 7692398
AN - SCOPUS:0027317070
SN - 0305-1048
VL - 21
SP - 4210
EP - 4217
JO - Nucleic acids research
JF - Nucleic acids research
IS - 18
ER -