HM2-phage resistant solventogenic Clostridium saccharoperbutylacetonicum N1-4 shows increased exopolysaccharide production

Xiaoji Liu, Barbara Turchi, Kenny C. Mok, Michiko E. Taga, Michael J Miller

Research output: Contribution to journalLetter

Abstract

During the history of the solventogenic clostridia fermentation industry, bacteriophages have been a recurrent problem. This study reports that HM2, a lytic bacteriophage for solventogenic Clostridium saccharoperbutylacetonicum N1-4 (N1-4), has a genome size of 17 470 bp with 22 open reading frames, including replication, lysis, integration and structural modules. To understand HM2 infection and resistance in N1-4, bacteriophage insensitive mutants (BIMs) were isolated and characterized. These eight independent BIMs included four adsorption mediated and four non-adsorption types. Adsorption mediated BIMs had increased exopolysaccharide (EPS) production and decreased attachment of HM2 to the cell surface. Non-adsorption mediated BIMs had a moderate increase in EPS production, which did not affect HM2 attachment. Absorption mediated BIMs had reduced fermentation performance, whereas the non-absorption mediated BIMs were indistinguishable from N1-4. Consequently, non-adsorption mediated BIMs would be more useful in a fermentation since they couple good fermentation performance with phage resistance.

Original languageEnglish (US)
Article numberfnx191
JournalFEMS Microbiology Letters
Volume364
Issue number19
DOIs
StatePublished - Oct 1 2017

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Clostridium
Bacteriophages
Fermentation
Adsorption
Genome Size
Open Reading Frames
Industry
History

Keywords

  • Bacteriophage
  • Bacteriophage insensitive mutants
  • Butanol
  • Clostridium
  • Exopolysaccharide

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

HM2-phage resistant solventogenic Clostridium saccharoperbutylacetonicum N1-4 shows increased exopolysaccharide production. / Liu, Xiaoji; Turchi, Barbara; Mok, Kenny C.; Taga, Michiko E.; Miller, Michael J.

In: FEMS Microbiology Letters, Vol. 364, No. 19, fnx191, 01.10.2017.

Research output: Contribution to journalLetter

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AU - Turchi, Barbara

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AU - Taga, Michiko E.

AU - Miller, Michael J

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N2 - During the history of the solventogenic clostridia fermentation industry, bacteriophages have been a recurrent problem. This study reports that HM2, a lytic bacteriophage for solventogenic Clostridium saccharoperbutylacetonicum N1-4 (N1-4), has a genome size of 17 470 bp with 22 open reading frames, including replication, lysis, integration and structural modules. To understand HM2 infection and resistance in N1-4, bacteriophage insensitive mutants (BIMs) were isolated and characterized. These eight independent BIMs included four adsorption mediated and four non-adsorption types. Adsorption mediated BIMs had increased exopolysaccharide (EPS) production and decreased attachment of HM2 to the cell surface. Non-adsorption mediated BIMs had a moderate increase in EPS production, which did not affect HM2 attachment. Absorption mediated BIMs had reduced fermentation performance, whereas the non-absorption mediated BIMs were indistinguishable from N1-4. Consequently, non-adsorption mediated BIMs would be more useful in a fermentation since they couple good fermentation performance with phage resistance.

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