TY - GEN
T1 - HIV-1 IIIB capture from whole blood on magnetic microparticles
AU - Damhorst, Gregory L.
AU - Kooiman, Jonathan M.
AU - Bashir, Rashid
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/10/13
Y1 - 2016/10/13
N2 - Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles functionalized with antibodies or proteins targeting components of the HIV envelope. Results show the best performance from T-20, a small peptide employed in antiretroviral pharmacotherapy which targets an HIV envelope protein. This demonstration introduces an interesting alternative to antibodies for future affinity-capture applications in HIV diagnostics.
AB - Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles functionalized with antibodies or proteins targeting components of the HIV envelope. Results show the best performance from T-20, a small peptide employed in antiretroviral pharmacotherapy which targets an HIV envelope protein. This demonstration introduces an interesting alternative to antibodies for future affinity-capture applications in HIV diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=85009072518&partnerID=8YFLogxK
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U2 - 10.1109/EMBC.2016.7592042
DO - 10.1109/EMBC.2016.7592042
M3 - Conference contribution
C2 - 28269569
AN - SCOPUS:85009072518
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 5785
EP - 5788
BT - 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
Y2 - 16 August 2016 through 20 August 2016
ER -