HIV-1 IIIB capture from whole blood on magnetic microparticles

Gregory L. Damhorst, Jonathan M. Kooiman, Rashid Bashir

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Viral load quantification is a critical need for HIV management worldwide. However, the diagnostic technologies currently available are too limited by their size and expense to reach many remote and resource-limited populations. Toward the development of techniques which can be leveraged for point-of-care assays, we have investigated affinity capture of whole viruses using magnetic microparticles functionalized with antibodies or proteins targeting components of the HIV envelope. Results show the best performance from T-20, a small peptide employed in antiretroviral pharmacotherapy which targets an HIV envelope protein. This demonstration introduces an interesting alternative to antibodies for future affinity-capture applications in HIV diagnostics.

Original languageEnglish (US)
Title of host publication2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages5785-5788
Number of pages4
ISBN (Electronic)9781457702204
DOIs
StatePublished - Oct 13 2016
Event38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016 - Orlando, United States
Duration: Aug 16 2016Aug 20 2016

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2016-October
ISSN (Print)1557-170X

Other

Other38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
CountryUnited States
CityOrlando
Period8/16/168/20/16

Fingerprint

Antibodies
HIV-1
Blood
HIV
Proteins
Drug therapy
Point-of-Care Systems
Viruses
Peptides
Human Immunodeficiency Virus Proteins
Assays
Antibody Affinity
Demonstrations
Protein Transport
Viral Load
Technology
Drug Therapy
Population

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Damhorst, G. L., Kooiman, J. M., & Bashir, R. (2016). HIV-1 IIIB capture from whole blood on magnetic microparticles. In 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016 (pp. 5785-5788). [7592042] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2016-October). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2016.7592042

HIV-1 IIIB capture from whole blood on magnetic microparticles. / Damhorst, Gregory L.; Kooiman, Jonathan M.; Bashir, Rashid.

2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 5785-5788 7592042 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2016-October).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Damhorst, GL, Kooiman, JM & Bashir, R 2016, HIV-1 IIIB capture from whole blood on magnetic microparticles. in 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016., 7592042, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, vol. 2016-October, Institute of Electrical and Electronics Engineers Inc., pp. 5785-5788, 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016, Orlando, United States, 8/16/16. https://doi.org/10.1109/EMBC.2016.7592042
Damhorst GL, Kooiman JM, Bashir R. HIV-1 IIIB capture from whole blood on magnetic microparticles. In 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 5785-5788. 7592042. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2016.7592042
Damhorst, Gregory L. ; Kooiman, Jonathan M. ; Bashir, Rashid. / HIV-1 IIIB capture from whole blood on magnetic microparticles. 2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 5785-5788 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).
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