Histidine availability alters glucagon gene expression in murine αTC6 cells

Gregory L. Paul, Aparna Waegner, H Rex Gaskins, Neil F. Shay

Research output: Contribution to journalArticle

Abstract

Because individual amino acids (AA) stimulate glucagon release from pancreatic α-cells, the purpose of this study was to determine if individual AA could influence glucagon gene expression. Preproglucagon mRNA levels were 67% lower (P < 0.05) in mouse αTC6 cells incubated for 12 h in amino acid- free medium compared with cells incubated in complete medium containing all 20 AA. A time-course study indicated that αTC6 cells incubated in amino acid-free medium ± 1 μmol/L puromycin or amino acid-containing medium plus puromycin exhibited similar preproglucagon mRNA decreases over 12 h. When 1 μmol/L actinomycin was added to medium with or without AA, ppG mRNA concentrations decreased (P < 0.05) for 3 h; however, values at 12 h were not different than those at 3 h. Deletions of single AA from complete medium demonstrated that only histidine removal or depletion reproduced the decrease in ppG mRNA observed in amino acid-free medium. We conclude that histidine is involved in the regulation of preproglucagon mRNA levels in αTC6 cells and that this regulation may be operative during both transcriptional and post- transcriptional events.

Original languageEnglish (US)
Pages (from-to)973-976
Number of pages4
JournalJournal of Nutrition
Volume128
Issue number6
StatePublished - Jun 1998

Fingerprint

glucagon
Glucagon
histidine
Histidine
Gene Expression
Amino Acids
gene expression
amino acids
mice
Proglucagon
free amino acids
puromycin
cells
Messenger RNA
Puromycin
Dactinomycin

Keywords

  • Aminoacids
  • Gene expression
  • Glucagon
  • Histidine
  • Mice

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Histidine availability alters glucagon gene expression in murine αTC6 cells. / Paul, Gregory L.; Waegner, Aparna; Gaskins, H Rex; Shay, Neil F.

In: Journal of Nutrition, Vol. 128, No. 6, 06.1998, p. 973-976.

Research output: Contribution to journalArticle

Paul, GL, Waegner, A, Gaskins, HR & Shay, NF 1998, 'Histidine availability alters glucagon gene expression in murine αTC6 cells', Journal of Nutrition, vol. 128, no. 6, pp. 973-976.
Paul, Gregory L. ; Waegner, Aparna ; Gaskins, H Rex ; Shay, Neil F. / Histidine availability alters glucagon gene expression in murine αTC6 cells. In: Journal of Nutrition. 1998 ; Vol. 128, No. 6. pp. 973-976.
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