Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling

Yi Chen, Zhida Xu, Gang Logan Liu, Jean Philippe Coppe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein-mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technology will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.

Original languageEnglish (US)
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages2295-2298
Number of pages4
DOIs
StatePublished - 2010
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: Nov 1 2010Nov 4 2010

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period11/1/1011/4/10

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Chen, Y., Xu, Z., Liu, G. L., & Coppe, J. P. (2010). Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling. In IEEE Sensors 2010 Conference, SENSORS 2010 (pp. 2295-2298). [5690802] https://doi.org/10.1109/ICSENS.2010.5690802