TY - GEN
T1 - Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling
AU - Chen, Yi
AU - Xu, Zhida
AU - Liu, Logan
AU - Coppe, Jean Philippe
PY - 2010
Y1 - 2010
N2 - Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein-mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technology will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.
AB - Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein-mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technology will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.
UR - http://www.scopus.com/inward/record.url?scp=79951930902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951930902&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5690802
DO - 10.1109/ICSENS.2010.5690802
M3 - Conference contribution
AN - SCOPUS:79951930902
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 2295
EP - 2298
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -