@inproceedings{d4a8a3d1880a4c0d904ef2a86c2f2816,
title = "Highly sensitive kinesin-microtubule motility assays using SLIM",
abstract = "We provide an experimental demonstration of Spatial Light Interference Microscopy (SLIM) as a tool for measuring the motion of 25 nm tubulin structures without the use of florescence labels. Compared to intensity imaging methods such as phase contrast or DIC, our imaging technique relies on the ratios of images associated with optically introduced phase shifts, thus implicitly removing background illumination. To demonstrate our new found capabilities, we characterize kinesin-based motility continuously over periods of time where fluorescence would typically photobleach. We exploit this new method to compare the motility of microtubules at low ATP concentrations, with and without the tagging proteins formerly required to perform these studies. Our preliminary results show that the tags have a non-negligible effect on the microtubule motility, slowing the process down by more than 10%.",
author = "Mikhail Kandel and Teng, {Kai Wen} and Selvin, {Paul R.} and Gabriel Popescu",
note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; 2nd Conference on Quantitative Phase Imaging, QPI 2016 ; Conference date: 14-02-2016 Through 17-02-2016",
year = "2016",
doi = "10.1117/12.2217109",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Gabriel Popescu and YongKeun Park",
booktitle = "Quantitative Phase Imaging II",
}