Higher protein intake during resistance training does not potentiate strength, but modulates gut microbiota, in middle-aged adults: a randomized control trial

Colleen F. Mckenna, Amadeo F. Salvador, Riley L Hughes, Susannah E. Scaroni, Rafael A. Alamilla, Andrew T Askow, Scott A. Paluska, Anna C Dilger, Hannah D. Holscher, Michael De Lisio, Naiman A Khan, Nicholas A. Burd

Research output: Contribution to journalArticlepeer-review

Abstract

Protein intake above the Recommended Dietary Allowance (RDA) and resistance training are known anabolic stimuli to support healthy aging. Specifically, protein supplementation after resistance exercise and nightly are strategies to maximize utilization of protein intake above the RDA in healthy adults. As such, the primary objective was to examine the efficacy of protein supplementation and nutritional counseling resulting in either moderate (MOD: ~1.0 g·kg−1·d−1) or higher (HIGH: ~1.6 g·kg−1·d−1) protein intake during resistance training on strength (one-repetition maximum, 1-RM; isokinetic and isometric peak torque) in healthy middle-aged adults. Exploratory analyses include diet-exercise effects on lean body mass (LBM), clinical biomarkers, gut microbiota, and diet composition. 50 middle-aged adults (age: 50 ± 8 y, BMI: 27.2 ± 4.1 kg·m-2) were randomized to either MOD or HIGH protein intake during a 10-week resistance training program (3 × week). Participants received dietary counseling and consumed either 15 g (MOD) or 30 g (HIGH) of protein from lean beef in the immediate post-exercise period and each evening. Maximal strength (1-RM) for all upper and lower body exercises significantly increased with no effect of protein intake (P<0.050). There was a main effect of time for LBM (P<0.005). Cardiovascular, renal, or glycemic biomarkers were not affected by the intervention. Gut microbiota were associated with several health outcomes (P<0.050). In conclusion, higher protein intake above moderate amounts does not potentiate resistance training adaptations in previously untrained middle-aged adults. This trial was registered at clinicaltrials.gov as NCT03029975.
Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
DOIs
StateAccepted/In press - Mar 8 2021

Keywords

  • hypertrophy
  • insulin resistance
  • gut microbiota
  • red meat

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