Higher order self-assembly of vesicles by site-specific binding

Shivkumar Chiruvolu, Scott Walker, Jacob Israelachvili, Franz Josef Schmitt, Deborah E Leckband, Joseph A. Zasadzinski

Research output: Contribution to journalArticle

Abstract

The association of lipid molecules into spherical vesicles in solution as a result of non-specific intermolecular forces constitutes a primary self- assembly process. Such vesicles can undergo a secondary self-assembly into higher order structures in a controlled and reversible manner by means of site-specific ligand-receptor (biotin-streptavidin) coupling. Cryoelectron microscopy shows these structures to be composed of tethered, rather than adhering, vesicles in their original, unstressed state. In contrast, vesicles aggregated by nonspecific, such as van der Waals, forces are deformed and stressed, producing unstable structures. Vesicle association by site-specific binding provides a practical mechanism for the production of stable, yet controllable, microstructured biomaterials.

Original languageEnglish (US)
Pages (from-to)1753-1756
Number of pages4
JournalScience
Volume264
Issue number5166
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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Cryoelectron Microscopy
Streptavidin
Biocompatible Materials
Binding Sites
Ligands
Lipids
biotin receptor

ASJC Scopus subject areas

  • General

Cite this

Chiruvolu, S., Walker, S., Israelachvili, J., Schmitt, F. J., Leckband, D. E., & Zasadzinski, J. A. (1994). Higher order self-assembly of vesicles by site-specific binding. Science, 264(5166), 1753-1756. https://doi.org/10.1126/science.8209255

Higher order self-assembly of vesicles by site-specific binding. / Chiruvolu, Shivkumar; Walker, Scott; Israelachvili, Jacob; Schmitt, Franz Josef; Leckband, Deborah E; Zasadzinski, Joseph A.

In: Science, Vol. 264, No. 5166, 01.01.1994, p. 1753-1756.

Research output: Contribution to journalArticle

Chiruvolu, S, Walker, S, Israelachvili, J, Schmitt, FJ, Leckband, DE & Zasadzinski, JA 1994, 'Higher order self-assembly of vesicles by site-specific binding', Science, vol. 264, no. 5166, pp. 1753-1756. https://doi.org/10.1126/science.8209255
Chiruvolu S, Walker S, Israelachvili J, Schmitt FJ, Leckband DE, Zasadzinski JA. Higher order self-assembly of vesicles by site-specific binding. Science. 1994 Jan 1;264(5166):1753-1756. https://doi.org/10.1126/science.8209255
Chiruvolu, Shivkumar ; Walker, Scott ; Israelachvili, Jacob ; Schmitt, Franz Josef ; Leckband, Deborah E ; Zasadzinski, Joseph A. / Higher order self-assembly of vesicles by site-specific binding. In: Science. 1994 ; Vol. 264, No. 5166. pp. 1753-1756.
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