High throughput screening and selection methods for directed enzyme evolution

Han Xiao, Zehua Bao, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated with significant and recent examples. These high throughput strategies are also discussed with an emphasis on compatibility with phenotypic analysis during directed enzyme evolution. Lastly, certain limitations of current methods, as well as future developments, are briefly summarized.

Original languageEnglish (US)
Pages (from-to)4011-4020
Number of pages10
JournalIndustrial and Engineering Chemistry Research
Volume54
Issue number16
DOIs
StatePublished - Apr 29 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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