Protein research is critical in fundamental biochemistry, drug development, and biocatalyst applications. High-throughput discovery, characterization, and engineering of proteins are highly desirable, considering the overwhelming availability of genomic information that awaits functional annotation, and the enormous structure-function space that can be explored using protein engineering strategies. While one can now create a large number of engineered proteins using a variety of microbial expression systems, the ability to chemically characterize them in a high throughput manner has lagged behind. Capable of label-free analysis that utilizes native substrates and ligands, mass spectrometry (MS) is well suited for high-throughput assays to characterize complex mixtures of engineered proteins. Here, we review recent advances that improve the throughput and information content of MS-based protein assays and describe notable applications that highlight these capabilities.

Original languageEnglish (US)
Title of host publicationProtein Engineering
Subtitle of host publicationTools and Applications
Number of pages23
ISBN (Electronic)9783527815128
ISBN (Print)9783527344703
StatePublished - Jan 1 2021

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'High-Throughput Mass Spectrometry Complements Protein Engineering'. Together they form a unique fingerprint.

Cite this