High throughput imaging of blood smears using white light diffraction phase microscopy

Hassaan Majeed, Mikhail E. Kandel, Basanta Bhaduri, Kevin Han, Zelun Luo, Krishnarao Tangella, Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

While automated blood cell counters have made great progress in detecting abnormalities in blood, the lack of specificity for a particular disease, limited information on single cell morphology and intrinsic uncertainly due to high throughput in these instruments often necessitates detailed inspection in the form of a peripheral blood smear. Such tests are relatively time consuming and frequently rely on medical professionals tally counting specific cell types. These assays rely on the contrast generated by chemical stains, with the signal intensity strongly related to staining and preparation techniques, frustrating machine learning algorithms that require consistent quantities to denote the features in question. Instead we opt to use quantitative phase imaging, understanding that the resulting image is entirely due to the structure (intrinsic contrast) rather than the complex interplay of stain and sample. We present here our first steps to automate peripheral blood smear scanning, in particular a method to generate the quantitative phase image of an entire blood smear at high throughput using white light diffraction phase microscopy (wDPM), a single shot and common path interferometric imaging technique.

Original languageEnglish (US)
Title of host publicationQuantitative Phase Imaging
EditorsYongKeun Park, Gabriel Popescu
PublisherSPIE
ISBN (Electronic)9781628414264
DOIs
StatePublished - Jan 1 2015
Event1st Conference on Quantitative Phase Imaging, QPI 2015 - San Francisco, United States
Duration: Feb 7 2015Feb 10 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9336
ISSN (Print)1605-7422

Other

Other1st Conference on Quantitative Phase Imaging, QPI 2015
CountryUnited States
CitySan Francisco
Period2/7/152/10/15

Keywords

  • hematology
  • interferometry
  • label-free imaging
  • microscopy
  • peripheral blood smear
  • quantitative phase imaging
  • wDPM

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'High throughput imaging of blood smears using white light diffraction phase microscopy'. Together they form a unique fingerprint.

  • Cite this

    Majeed, H., Kandel, M. E., Bhaduri, B., Han, K., Luo, Z., Tangella, K., & Popescu, G. (2015). High throughput imaging of blood smears using white light diffraction phase microscopy. In Y. Park, & G. Popescu (Eds.), Quantitative Phase Imaging [93361Y] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9336). SPIE. https://doi.org/10.1117/12.2080200