High-throughput force measurement of individual kinesin-1 motors during multi-motor transport

Saurabh Shukla, Alice Troitskaia, Nikhila Swarna, Barun Kumar Maity, Marco Tjioe, Carol S. Bookwalter, Kathleen M. Trybus, Yann R. Chemla, Paul R. Selvin

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular motors often work in teams to move a cellular cargo. Yet measuring the forces exerted by each motor is challenging. Using a sensor made with denatured ssDNA and multi-color fluorescence, we measured picoNewtons of forces and nanometer distances exerted by individual constrained kinesin-1 motors acting together while driving a common microtubule in vitro. We find that kinesins primarily exerted less than 1 pN force, even while the microtubule is bypassing artificial obstacles of 20-100 nanometer size. Occasionally, individual forces increase upon encountering obstacles, although at other times they do not, with the cargo continuing in a directional manner. Our high-throughput technique, which can measure forces by many motors simultaneously, is expected to be useful for many different types of molecular motors.

Original languageEnglish (US)
Pages (from-to)12463-12475
Number of pages13
JournalNanoscale
Volume14
Issue number34
DOIs
StatePublished - Aug 15 2022

ASJC Scopus subject areas

  • Materials Science(all)

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