Ferulic acid is a hydroxycinnamic acid that possesses positive health benefits and plays a role in both pathogen and insect resistance in maize (Zea mays L.). Many protocols for the extraction of insoluble-bound ferulic acid in maize use methods that are low-throughput and are not conducive to analyzing the large number of samples used in field research or breeding programs. We examined two protocols for the extraction of insoluble-bound ferulic acid that use small sample sizes typical of experiments where a large number of samples need to be analyzed. The main distinguishing feature between the two protocols is the inclusion of a starch digestion step in Protocol A to inhibit the swelling of intact starch when introduced to an alkali reagent. Our results indicate that the inclusion of a starch digestion step during the extraction of insoluble-bound ferulic acid from maize is necessary if small sample sizes are used. The omission of the starch digestion step in Protocol B resulted in an average percent recovery of only 79.0% of the insoluble-bound ferulic acid. Conversely, the inclusion of the starch digestion step in Protocol A allowed for the accurate and high-throughput extraction of insoluble-bound ferulic acid at lower cost than Protocol B.
ASJC Scopus subject areas
- Agronomy and Crop Science