High-throughput extraction of insoluble-bound ferulic acid in maize

Carrie J. Butts-Wilmsmeyer, Martin O. Bohn

Research output: Contribution to journalArticlepeer-review

Abstract

Ferulic acid is a hydroxycinnamic acid that possesses positive health benefits and plays a role in both pathogen and insect resistance in maize (Zea mays L.). Many protocols for the extraction of insoluble-bound ferulic acid in maize use methods that are low-throughput and are not conducive to analyzing the large number of samples used in field research or breeding programs. We examined two protocols for the extraction of insoluble-bound ferulic acid that use small sample sizes typical of experiments where a large number of samples need to be analyzed. The main distinguishing feature between the two protocols is the inclusion of a starch digestion step in Protocol A to inhibit the swelling of intact starch when introduced to an alkali reagent. Our results indicate that the inclusion of a starch digestion step during the extraction of insoluble-bound ferulic acid from maize is necessary if small sample sizes are used. The omission of the starch digestion step in Protocol B resulted in an average percent recovery of only 79.0% of the insoluble-bound ferulic acid. Conversely, the inclusion of the starch digestion step in Protocol A allowed for the accurate and high-throughput extraction of insoluble-bound ferulic acid at lower cost than Protocol B.

Original languageEnglish (US)
Pages (from-to)3152-3159
Number of pages8
JournalCrop Science
Volume56
Issue number6
DOIs
StatePublished - Nov 1 2016

ASJC Scopus subject areas

  • Agronomy and Crop Science

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