High throughput cytotoxicity screening using photonic crystal biosensors

Leo L. Chan, Saujanya L. Gosangari, Kenneth L. Watkin, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages799-802
Number of pages4
DOIs
StatePublished - Dec 1 2007
Event4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
Duration: Jun 10 2007Jun 14 2007

Publication series

NameTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
CountryFrance
CityLyon
Period6/10/076/14/07

Fingerprint

Cytotoxicity
Photonic crystals
Biosensors
Screening
Throughput
Wave filters
Cell proliferation
Cell death
Bioactivity
Labels
Cells
Wavelength
Sensors

Keywords

  • Apoptosis
  • Cell-based assay
  • Label-free
  • Microplate
  • Photonic crystal

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Chan, L. L., Gosangari, S. L., Watkin, K. L., & Cunningham, B. T. (2007). High throughput cytotoxicity screening using photonic crystal biosensors. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 799-802). [4300251] (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300251

High throughput cytotoxicity screening using photonic crystal biosensors. / Chan, Leo L.; Gosangari, Saujanya L.; Watkin, Kenneth L.; Cunningham, Brian T.

TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 799-802 4300251 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chan, LL, Gosangari, SL, Watkin, KL & Cunningham, BT 2007, High throughput cytotoxicity screening using photonic crystal biosensors. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300251, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 799-802, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 6/10/07. https://doi.org/10.1109/SENSOR.2007.4300251
Chan LL, Gosangari SL, Watkin KL, Cunningham BT. High throughput cytotoxicity screening using photonic crystal biosensors. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 799-802. 4300251. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300251
Chan, Leo L. ; Gosangari, Saujanya L. ; Watkin, Kenneth L. ; Cunningham, Brian T. / High throughput cytotoxicity screening using photonic crystal biosensors. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 799-802 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).
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