Abstract
A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.
| Original language | English (US) |
|---|---|
| Title of host publication | TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems |
| Pages | 799-802 |
| Number of pages | 4 |
| DOIs | |
| State | Published - Dec 1 2007 |
| Event | 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France Duration: Jun 10 2007 → Jun 14 2007 |
Publication series
| Name | TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems |
|---|
Other
| Other | 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 |
|---|---|
| Country | France |
| City | Lyon |
| Period | 6/10/07 → 6/14/07 |
Fingerprint
Keywords
- Apoptosis
- Cell-based assay
- Label-free
- Microplate
- Photonic crystal
ASJC Scopus subject areas
- Control and Systems Engineering
- Electrical and Electronic Engineering
Cite this
High throughput cytotoxicity screening using photonic crystal biosensors. / Chan, Leo L.; Gosangari, Saujanya L.; Watkin, Kenneth L.; Cunningham, Brian T.
TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 799-802 4300251 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - High throughput cytotoxicity screening using photonic crystal biosensors
AU - Chan, Leo L.
AU - Gosangari, Saujanya L.
AU - Watkin, Kenneth L.
AU - Cunningham, Brian T.
PY - 2007/12/1
Y1 - 2007/12/1
N2 - A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.
AB - A label-free method for detecting the attachment of human cancer cells to a biosensor surface for rapid screening for biological activity is described, in which attachment of a cell results in highly localized increase of the resonant reflected wavelength of a photonic crystal narrowband reflectance filter incorporated into a standard 96-well microplate. We demonstrate the efficacy of this method as an early screening technique for the rapid quantification of the rate of cancer cell proliferation on the sensor surface, and subsequently as a means for quantifying cell detachment resulting from apoptosis that is induced by exposure of the cells to cytotoxic chemicals.
KW - Apoptosis
KW - Cell-based assay
KW - Label-free
KW - Microplate
KW - Photonic crystal
UR - http://www.scopus.com/inward/record.url?scp=50049135191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049135191&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300251
DO - 10.1109/SENSOR.2007.4300251
M3 - Conference contribution
AN - SCOPUS:50049135191
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 799
EP - 802
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
ER -