Abstract
A relatively new technique termed colony blot hybridization appears to be a reliable replacement for the ligated porcine gut loop assay previously used to detect stable toxin-B producing Escherichia coli. The highly reproducible blot assay enables screening of large numbers of isolants for the presence of E coli stable-B toxin genes, thus avoiding variations in phenotypic expression and poor repeatability inherent in the in vivo assays. Availability of this diagnostic test can aid practitioners in identifying enterotoxigenic E coli related disease in swine and in determining the benefits of vaccination programs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 57-59 |
| Number of pages | 3 |
| Journal | Journal of the American Veterinary Medical Association |
| Volume | 188 |
| Issue number | 1 |
| State | Published - Jan 1 1986 |
| Externally published | Yes |
ASJC Scopus subject areas
- veterinary(all)