High technology diagnostics: detection of enterotoxigenic Escherichia coli, using DNA probes.

C. W. Maddox, R. A. Wilson

Research output: Contribution to journalArticlepeer-review

Abstract

A relatively new technique termed colony blot hybridization appears to be a reliable replacement for the ligated porcine gut loop assay previously used to detect stable toxin-B producing Escherichia coli. The highly reproducible blot assay enables screening of large numbers of isolants for the presence of E coli stable-B toxin genes, thus avoiding variations in phenotypic expression and poor repeatability inherent in the in vivo assays. Availability of this diagnostic test can aid practitioners in identifying enterotoxigenic E coli related disease in swine and in determining the benefits of vaccination programs.

Original languageEnglish (US)
Pages (from-to)57-59
Number of pages3
JournalJournal of the American Veterinary Medical Association
Volume188
Issue number1
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • General Veterinary

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