High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging

Andrew J. Bower, Joanne Li, Eric J. Chaney, Marina Marjanovic, Stephen Allen Boppart

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Two-photon fluorescence lifetime imaging microscopy (TP-FLIM) of reduced nicotinamide adenine dinucleotide is a sensitive metabolic imaging modality. Here, a video rate TP-FLIM system is demonstrated for metabolic imaging in cells and tissues.

Original languageEnglish (US)
Title of host publicationOptical Molecular Probes, Imaging and Drug Delivery, OMP 2017
PublisherOSA - The Optical Society
VolumePart F63-OMP 2017
ISBN (Electronic)9781557528209
DOIs
StatePublished - 2017
EventOptical Molecular Probes, Imaging and Drug Delivery, OMP 2017 - San Diego, United States
Duration: Apr 2 2017 → Apr 5 2017

Other

OtherOptical Molecular Probes, Imaging and Drug Delivery, OMP 2017
CountryUnited States
CitySan Diego
Period4/2/17 → 4/5/17

Fingerprint

NAD
Labels
Microscopic examination
Photons
Fluorescence
Imaging techniques
Tissue

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

Cite this

Bower, A. J., Li, J., Chaney, E. J., Marjanovic, M., & Boppart, S. A. (2017). High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. In Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017 (Vol. Part F63-OMP 2017). OSA - The Optical Society. https://doi.org/10.1364/OMP.2017.OmS2D.5

High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. / Bower, Andrew J.; Li, Joanne; Chaney, Eric J.; Marjanovic, Marina; Boppart, Stephen Allen.

Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. Vol. Part F63-OMP 2017 OSA - The Optical Society, 2017.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Bower, AJ, Li, J, Chaney, EJ, Marjanovic, M & Boppart, SA 2017, High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. in Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. vol. Part F63-OMP 2017, OSA - The Optical Society, Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017, San Diego, United States, 4/2/17. https://doi.org/10.1364/OMP.2017.OmS2D.5
Bower AJ, Li J, Chaney EJ, Marjanovic M, Boppart SA. High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. In Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. Vol. Part F63-OMP 2017. OSA - The Optical Society. 2017 https://doi.org/10.1364/OMP.2017.OmS2D.5
Bower, Andrew J. ; Li, Joanne ; Chaney, Eric J. ; Marjanovic, Marina ; Boppart, Stephen Allen. / High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. Vol. Part F63-OMP 2017 OSA - The Optical Society, 2017.
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