High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging

Andrew J. Bower; Joanne Li; Eric J. Chaney; Marina Marjanovic; Stephen A. Boppart

doi:10.1364/OMP.2017.OmS2D.5

High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging

Andrew J. Bower, Joanne Li, Eric J. Chaney, Marina Marjanovic, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Two-photon fluorescence lifetime imaging microscopy (TP-FLIM) of reduced nicotinamide adenine dinucleotide is a sensitive metabolic imaging modality. Here, a video rate TP-FLIM system is demonstrated for metabolic imaging in cells and tissues.

Original language	English (US)
Title of host publication	Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017
Publisher	OSA - The Optical Society
ISBN (Electronic)	9781557528209
DOIs	https://doi.org/10.1364/OMP.2017.OmS2D.5
State	Published - Jan 1 2017
Event	Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017 - San Diego, United States Duration: Apr 2 2017 → Apr 5 2017

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F63-OMP 2017

Other

Other	Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017
Country	United States
City	San Diego
Period	4/2/17 → 4/5/17

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ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

Cite this

Bower, A. J., Li, J., Chaney, E. J., Marjanovic, M., & Boppart, S. A. (2017). High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. In Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017 (Optics InfoBase Conference Papers; Vol. Part F63-OMP 2017). OSA - The Optical Society. https://doi.org/10.1364/OMP.2017.OmS2D.5

High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. / Bower, Andrew J.; Li, Joanne; Chaney, Eric J.; Marjanovic, Marina ; Boppart, Stephen A.

Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. OSA - The Optical Society, 2017. (Optics InfoBase Conference Papers; Vol. Part F63-OMP 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Bower, AJ, Li, J, Chaney, EJ, Marjanovic, M & Boppart, SA 2017, High-speed two-photon fluorescence lifetime imaging microscopy of NADH for label-free metabolic imaging. in Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017. Optics InfoBase Conference Papers, vol. Part F63-OMP 2017, OSA - The Optical Society, Optical Molecular Probes, Imaging and Drug Delivery, OMP 2017, San Diego, United States, 4/2/17. https://doi.org/10.1364/OMP.2017.OmS2D.5

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Access to Document

10.1364/OMP.2017.OmS2D.5