High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells

Yayao Ma, Youngjae Lee, Catherine Best-Popescu, Liang Gao

Research output: Contribution to journalArticlepeer-review

Abstract

We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.

Original languageEnglish (US)
Article numbere2004176118
JournalProceedings of the National Academy of Sciences of the United States of America
Volume118
Issue number3
DOIs
StatePublished - Jan 19 2021

Keywords

  • Compressed imaging
  • FLIM
  • High-speed

ASJC Scopus subject areas

  • General

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