Abstract
A novel process applying high solids loading in chemical-free pretreatment and enzymatic hydrolysis was developed to produce sugars from bioenergy sorghum. Hydrothermal pretreatment with 50% solids loading was performed in a pilot scale continuous reactor followed by disc refining. Sugars were extracted from the enzymatic hydrolysis at 10% to 50% solids content using fed-batch operations. Three surfactants (Tween 80, PEG 4000, and PEG 6000) were evaluated to increase sugar yields. Hydrolysis using 2% PEG 4000 had the highest sugar yields. Glucose concentrations of 105, 130, and 147 g/L were obtained from the reaction at 30%, 40%, and 50% solids content, respectively. The maximum sugar concentration of the hydrolysate, including glucose and xylose, obtained was 232 g/L. Additionally, the glucose recovery (73.14%) was increased compared to that of the batch reaction (52.74%) by using two-stage enzymatic hydrolysis combined with fed-batch operation at 50% w/v solids content.
Original language | English (US) |
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Article number | 124051 |
Journal | Bioresource Technology |
Volume | 318 |
DOIs | |
State | Published - Dec 2020 |
Keywords
- Bioenergy sorghum
- Cellulosic sugars
- Enzymatic hydrolysis
- High solids process
- Hydrothermal pretreatment
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal