High solids loading biorefinery for the production of cellulosic sugars from bioenergy sorghum

Ming Hsun Cheng, Haider Jawad Kadhum, Ganti S. Murthy, Bruce S. Dien, Vijay Singh

Research output: Contribution to journalArticlepeer-review


A novel process applying high solids loading in chemical-free pretreatment and enzymatic hydrolysis was developed to produce sugars from bioenergy sorghum. Hydrothermal pretreatment with 50% solids loading was performed in a pilot scale continuous reactor followed by disc refining. Sugars were extracted from the enzymatic hydrolysis at 10% to 50% solids content using fed-batch operations. Three surfactants (Tween 80, PEG 4000, and PEG 6000) were evaluated to increase sugar yields. Hydrolysis using 2% PEG 4000 had the highest sugar yields. Glucose concentrations of 105, 130, and 147 g/L were obtained from the reaction at 30%, 40%, and 50% solids content, respectively. The maximum sugar concentration of the hydrolysate, including glucose and xylose, obtained was 232 g/L. Additionally, the glucose recovery (73.14%) was increased compared to that of the batch reaction (52.74%) by using two-stage enzymatic hydrolysis combined with fed-batch operation at 50% w/v solids content.

Original languageEnglish (US)
Article number124051
JournalBioresource Technology
StatePublished - Dec 2020


  • Bioenergy sorghum
  • Cellulosic sugars
  • Enzymatic hydrolysis
  • High solids process
  • Hydrothermal pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


Dive into the research topics of 'High solids loading biorefinery for the production of cellulosic sugars from bioenergy sorghum'. Together they form a unique fingerprint.

Cite this