High-Resolution Secondary Ion Mass Spectrometry Analysis of Cell Membranes

Brittney L. Gorman, Mary L. Kraft

Research output: Contribution to journalReview articlepeer-review


This Feature describes the use a Cameca NanoSIMS instrument for directly imaging specific lipid and protein species in the plasma membranes of mammalian cells with approximately 100 nm-lateral resolution and discusses how these analyses have already begun to transform fundamental concepts in the field of membrane biology. Secondary ion generation is discussed with emphasis on the constraints that affect the detection and identification of membrane components, and then the sample preparation methodologies and data analysis strategies that address these constraints are described.

Original languageEnglish (US)
Pages (from-to)1645-1652
Number of pages8
JournalAnalytical Chemistry
Issue number2
StatePublished - Jan 21 2020

ASJC Scopus subject areas

  • Analytical Chemistry


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