@inbook{a409a255878149168d3723d7ae4ddbb7,
title = "High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy",
abstract = "We describe the design, construction, and application of an instrument combining dual-trap, high-resolution optical tweezers and a confocal microscope. This hybrid instrument allows nanomechanical manipulation and measurement simultaneously with single-molecule fluorescence detection. We present the general design principles that overcome the challenges of maximizing optical trap resolution while maintaining single-molecule fluorescence sensitivity, and provide details on the construction and alignment of the instrument. This powerful new tool is just beginning to be applied to biological problems. We present step-by-step instructions on an application of this technique that highlights the instrument's capabilities, detecting conformational dynamics in a nucleic acid-processing enzyme.",
keywords = "Confocal microscopy, FRET, F{\"o}rster Resonance Energy Transfer, Helicase, Optical trapping, Optical tweezers, Single-molecule fluorescence, UvrD",
author = "Whitley, {K. D.} and Comstock, {M. J.} and Chemla, {Y. R.}",
note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
doi = "10.1016/bs.mie.2016.10.036",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "137--169",
booktitle = "Methods in Enzymology",
address = "United States",
}