High-resolution mid-infrared imaging for disease diagnosis

Michael J. Walsh, David Mayerich, Andre Kajdacsy-Balla, Rohit Bhargava

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Histopathology is the gold standard for disease diagnosis; however it is subject to a number of limitations. Fourier Transform infrared (FT-IR) spectroscopic imaging can be used to derive chemical images from tissues based on their inherent molecular composition, thereby eliminating the use of dyes and stains. FT-IR imaging represents a novel, emerging approach that can allow for accurate cell type identification which is competitive with conventional histopathological approaches and may alleviate a number of the limitations associated with current techniques. Traditionally, this approach has involved in a loss of image detail due to the sub-optimal and, compared to optical microscopy, coarse pixel size in instruments. Recent advances in high-resolution FT-IR imaging have allowed for the identification and chemical characterization of cell types and tissue structures which were previously not discernible. Here we report on the visualization of several histologic details using high-resolution IR imaging that may be critical for tissue histology and disease diagnosis.

Original languageEnglish (US)
Title of host publicationBiomedical Vibrational Spectroscopy V
Subtitle of host publicationAdvances in Research and Industry
DOIs
StatePublished - Jun 11 2012
EventBiomedical Vibrational Spectroscopy V: Advances in Research and Industry - San Francisco, CA, United States
Duration: Jan 21 2012Jan 22 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8219
ISSN (Print)1605-7422

Other

OtherBiomedical Vibrational Spectroscopy V: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period1/21/121/22/12

Keywords

  • Colon
  • Fourier Transform Infrared
  • Imaging
  • Spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Walsh, M. J., Mayerich, D., Kajdacsy-Balla, A., & Bhargava, R. (2012). High-resolution mid-infrared imaging for disease diagnosis. In Biomedical Vibrational Spectroscopy V: Advances in Research and Industry [82190R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8219). https://doi.org/10.1117/12.909339