High-resolution dual-trap optical tweezers with differential detection: Data collection and instrument calibration

Carlos Bustamante; Yann R. Chemla; Jeffrey R. Moffitt

doi:10.1101/pdb.ip74

High-resolution dual-trap optical tweezers with differential detection: Data collection and instrument calibration

Carlos Bustamante, Yann R. Chemla, Jeffrey R. Moffitt

Research output: Contribution to journal › Article › peer-review

Abstract

Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.

Original language	English (US)
Journal	Cold Spring Harbor protocols
Volume	4
Issue number	10
DOIs	https://doi.org/10.1101/pdb.ip74
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/pdb.ip74

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AB - Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.

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High-resolution dual-trap optical tweezers with differential detection: Data collection and instrument calibration

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