High-resolution dual-trap optical tweezers with differential detection: Data collection and instrument calibration

Carlos Bustamante, Yann R. Chemla, Jeffrey R. Moffitt

Research output: Contribution to journalArticle

Abstract

Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.

Original languageEnglish (US)
JournalCold Spring Harbor protocols
Volume4
Issue number10
DOIs
StatePublished - Dec 7 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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