High-pressure treatment of polytene chromosomes improves structural resolution

Dmitri V. Novikov, Igor Kireev, Andrew S. Belmont

Research output: Contribution to journalArticlepeer-review

Abstract

The exceptional cytology provided by polytene chromosomes has made Drosophila melanogaster a premier model for chromosome studies, but full exploitation of polytene cytology is impeded by the difficulty in preparing high-quality chromosome spreads. Here we describe use of high pressure to produce formaldehyde-fixed chromosome spreads, which upon light-microscopy examination reveal structural detail previously observed only in electron microscopy preparations. We demonstrate applications to immunofluorescence and in situ hybridization.

Original languageEnglish (US)
Pages (from-to)483-485
Number of pages3
JournalNature Methods
Volume4
Issue number6
DOIs
StatePublished - Jun 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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