High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field

Darshi Banan, Rachel E. Paul, Max J. Feldman, Mark W. Holmes, Hannah Schlake, Ivan Baxter, Hui Jiang, Andrew D.B. Leakey

Research output: Contribution to journalArticle

Abstract

Field-based, rapid, and nondestructive techniques for assessing plant productivity are needed to accelerate the discovery of genotype-to-phenotype relationships in next-generation biomass grass crops. The use of hemispherical imaging and light attenuation modeling was evaluated against destructive harvest measures with respect to their ability to accurately capture phenotypic and genotypic relationships in a field-grown grass crop. Plant area index (PAI) estimated from below-canopy hemispherical images, as well as a suite of thirteen traits assessed by manual destructive harvests, were measured in a Setaria recombinant inbred line mapping population segregating for aboveground productivity and architecture. A significant correlation was observed between PAI and biomass production across the population at maturity (r 2  =.60), as well as for select diverse genotypes sampled repeatedly over the growing season (r 2  =.79). Twenty-seven quantitative trait loci (QTL) were detected for manually collected traits associated with biomass production. Of these, twenty-one were found in four clusters of colocalized QTL. Analysis of image-based estimates of PAI successfully identified all four QTL hot spots for biomass production. QTL for PAI had greater overlap with those detected for traits associated with biomass production than with those for plant architecture and biomass partitioning. Hemispherical imaging is an affordable and scalable method, which demonstrates how high-throughput phenotyping can identify QTL related to biomass production of field trials in place of destructive harvests that are labor, time, and material intensive.

Original languageEnglish (US)
Article numbere00041
JournalPlant Direct
Volume2
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Quantitative Trait Loci
Biomass
Crops
biomass production
quantitative trait loci
image analysis
Imaging techniques
Costs and Cost Analysis
crop
biomass
cost
Costs
Poaceae
Setaria (Poaceae)
grasses
genotype
phenotype
grass
plant architecture
Productivity

Keywords

  • Leaf Area Index
  • crop production
  • hemispherical photographs
  • high-throughput phenotyping
  • setaria

ASJC Scopus subject areas

  • Plant Science
  • Ecology
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field. / Banan, Darshi; Paul, Rachel E.; Feldman, Max J.; Holmes, Mark W.; Schlake, Hannah; Baxter, Ivan; Jiang, Hui; Leakey, Andrew D.B.

In: Plant Direct, Vol. 2, No. 2, e00041, 01.02.2018.

Research output: Contribution to journalArticle

Banan, D, Paul, RE, Feldman, MJ, Holmes, MW, Schlake, H, Baxter, I, Jiang, H & Leakey, ADB 2018, 'High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field', Plant Direct, vol. 2, no. 2, e00041. https://doi.org/10.1002/pld3.41
Banan D, Paul RE, Feldman MJ, Holmes MW, Schlake H, Baxter I et al. High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field. Plant Direct. 2018 Feb 1;2(2). e00041. https://doi.org/10.1002/pld3.41
Banan, Darshi ; Paul, Rachel E. ; Feldman, Max J. ; Holmes, Mark W. ; Schlake, Hannah ; Baxter, Ivan ; Jiang, Hui ; Leakey, Andrew D.B. / High-fidelity detection of crop biomass quantitative trait loci from low-cost imaging in the field. In: Plant Direct. 2018 ; Vol. 2, No. 2.
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