@inbook{ed8e7ea2abd2446f8948ea4187b7537f,
title = "High-Efficiency Genome Editing of Streptomyces Species by an Engineered CRISPR/Cas System",
abstract = "Next-generation sequencing technologies have rapidly expanded the genomic information of numerous organisms and revealed a rich reservoir of natural product gene clusters from microbial genomes, especially from Streptomyces, the largest genus of known actinobacteria at present. However, genetic engineering of these bacteria is often time consuming and labor intensive, if even possible. In this chapter, we describe the design and construction of pCRISPomyces, an engineered Type II CRISPR/Cas system, for targeted multiplex gene deletions in Streptomyces lividans, Streptomyces albus, and Streptomyces viridochromogenes with editing efficiency ranging from 70\% to 100\%. We demonstrate pCRISPomyces as a powerful tool for genome editing in Streptomyces.",
keywords = "CRISPR, Genome engineering, Natural product biosynthesis, Streptomyces, Synthetic biology",
author = "Y. Wang and Cobb, \{R. E.\} and H. Zhao",
note = "This work was supported by the National Institutes of Health (GM077596).",
year = "2016",
doi = "10.1016/bs.mie.2016.03.014",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "271--284",
editor = "O'Connor, \{Sarah E.\}",
booktitle = "Methods in Enzymology",
address = "United States",
}