High-definition fourier transform infrared spectroscopic imaging of breast tissue

L. Suzanne Leslie, Andre Kadjacsy-Balla, Rohit Bhargava

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Breast cancer diagnosis relies on staining serial sections of a biopsy in a process that can be time intensive and costly. Fourier transform infrared imaging (FT-IR) is a non-destructive, label-free chemical imaging technique that uses the vibrational structure of the biological molecules of the sample to provide contrast for images at any absorption peak in the mid-infrared. The full potential of spectroscopic imaging has been limited by the spatial resolution provided by most commercial instruments. By increasing the magnification and numerical aperture of the microscope, image pixel sizes on the order of 1.1 micron can be achieved, allowing HD FT-IR spectroscopic imaging to provide high quality images that could aid in histopathology, diagnosis, and studies of breast cancer progression.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2015
Subtitle of host publicationDigital Pathology
EditorsMetin N. Gurcan, Anant Madabhushi
ISBN (Electronic)9781628415100
StatePublished - 2015
EventMedical Imaging 2015: Digital Pathology - Orlando, United States
Duration: Feb 25 2015Feb 26 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMedical Imaging 2015: Digital Pathology
Country/TerritoryUnited States


  • Breast
  • FT-IR
  • High definition
  • Magnification
  • Numerical aperture
  • Resolution
  • SNR
  • Spectroscopic imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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