High affinity TCR proteins and methods

Phillip D Holler (Inventor), David M Kranz (Inventor), Karl Dane Wittrup (Inventor)

Research output: Patent

Abstract

T cell receptors (TCRS) that have higher affinity for a ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody.
Original languageEnglish (US)
U.S. patent number7569357
StatePublished - Aug 4 2009

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