Hierarchical oligonucleotide primer extension (HOPE)-based approach to identify sources of fecal contamination in water bodies

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A set of HOPE primers to identify human and nonhuman fecal contamination ○5 primers to detect human-associated Bacteroides, 5 primers to detect dog-, pig- and cow-specific Bacteroides Tested on 62 feces collected from known origins, formulated a set evaluation criteria ○ All four human-associated Bacteroides present = human feces ○ Animal-specific primer present = animal feces ○ Spatial location of sample in MDS plot Test 1: 21 blind samples ○Able to correctly identify fecal origins in 81% of blind samples ○Remaining samples have inconclusive results because of low abundances of dog-specific Bacteroidales Test 2: environmental waters 1.Able to indicate impaired water qualities, results comparable to fecal coliform test 2.Able to denote fecal origins, provides an advantage over fecal coliform test. 2009

Original languageEnglish (US)
Title of host publicationWater Quality Technology Conference and Exposition 2009
Pages1713-1722
Number of pages10
StatePublished - 2009
EventWater Quality Technology Conference and Exposition 2009 - Seattle, WA, United States
Duration: Nov 15 2009Nov 19 2009

Other

OtherWater Quality Technology Conference and Exposition 2009
Country/TerritoryUnited States
CitySeattle, WA
Period11/15/0911/19/09

ASJC Scopus subject areas

  • Management, Monitoring, Policy and Law
  • Water Science and Technology

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