Heterologous production of the lantibiotic ala(0)actagardine in escherichia coli

Yanxiang Shi; Alejandro Bueno; Wilfred A. Vander Donk

doi:10.1039/c2cc36336d

Heterologous production of the lantibiotic ala(0)actagardine in escherichia coli

Yanxiang Shi, Alejandro Bueno, Wilfred A. Vander Donk

Research output: Contribution to journal › Article › peer-review

Abstract

We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.

Original language	English (US)
Pages (from-to)	10966-10968
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	89
DOIs	https://doi.org/10.1039/c2cc36336d
State	Published - Oct 15 2012

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.",

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AB - We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.

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Heterologous production of the lantibiotic ala(0)actagardine in escherichia coli

Abstract

ASJC Scopus subject areas

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