TY - JOUR
T1 - Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa
AU - Woodyer, Ryan
AU - Simurdiak, Michael
AU - Van Der Donk, Wilfred A.
AU - Zhao, Huimin
PY - 2005/3
Y1 - 2005/3
N2 - A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.
AB - A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.
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U2 - 10.1128/AEM.71.3.1642-1647.2005
DO - 10.1128/AEM.71.3.1642-1647.2005
M3 - Article
C2 - 15746370
AN - SCOPUS:15444371063
SN - 0099-2240
VL - 71
SP - 1642
EP - 1647
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 3
ER -