Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa

Ryan Woodyer, Michael Simurdiak, Wilfred A. Van Der Donk, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

Original languageEnglish (US)
Pages (from-to)1642-1647
Number of pages6
JournalApplied and environmental microbiology
Volume71
Issue number3
DOIs
StatePublished - Mar 2005

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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