Heterologous expression of endo-β-1,4-D-glucanase from Clostridium cellulovorans in Clostridium acetobutylicum ATCC 824 following transformation of the engB gene

A. Y. Kim, G. T. Attwood, S. M. Holt, Bryan A White, H. P. Blaschek

Research output: Contribution to journalArticlepeer-review

Abstract

Heterologous expression of the Clostridium cellulovorans engB gene by Clostridium acetobutylicum BKW-1 was detected as zones of hydrolysis on carboxymethyl cellulose (CMC) Trypticase glucose yeast plates stained with Congo red. The extracellular cellulase preparation from C. acetobutylicum BKW-1 has a specific activity towards CMC which is more than fourfold that present in C. acetobutylicum ATCC 824. Western blot (immunoblot) analysis using the C. cellulovorans anti-EngB primary antibody demonstrated that an additional 44-kDa protein band was present in the supernatant derived from C. acetobutylicum BKW-1 but was not present in ATCC 824 or ATCC 824 (pMTL500E).

Original languageEnglish (US)
Pages (from-to)337-340
Number of pages4
JournalApplied and Environmental Microbiology
Volume60
Issue number1
StatePublished - 1994

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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