Heterogeneity of vaginal microbial communities within individuals

Tae Kyung Kim, Susan M. Thomas, Mengfei Ho, Shobha Sharma, Claudia I. Reich, Jeremy A. Frank, Kathleen M. Yeater, Diana R. Biggs, Noriko Nakamura, Rebecca Stumpf, Steven R. Leigh, Richard I. Tapping, Steven R. Blanke, James M. Slauch, H. Rex Gaskins, Jon S. Weisbaum, Gary J. Olsen, Lois L. Hoyer, Brenda A. Wilson

Research output: Contribution to journalArticle

Abstract

Recent culture-independent studies have revealed that a healthy vaginal ecosystem harbors a surprisingly complex assemblage of microorganisms. However, the spatial distribution and composition of vaginal microbial populations have not been investigated using molecular methods. Here, we evaluated site-specific microbial composition within the vaginal ecosystem and examined the influence of sampling technique in detection of the vaginal microbiota. 16S rRNA gene clone libraries were prepared from samples obtained from different locations (cervix, fornix, outer vaginal canal) and by different methods (swabbing, scraping, lavaging) from the vaginal tracts of eight clinically healthy, asymptomatic women. The data reveal that the vaginal microbiota is not homogenous throughout the vaginal tract but differs significantly within an individual with regard to anatomical site and sampling method used. Thus, this study illuminates the complex structure of the vaginal ecosystem and calls for the consideration of microenvironments when sampling vaginal microbiota as a clinical predictor of vaginal health.

Original languageEnglish (US)
Pages (from-to)1181-1189
Number of pages9
JournalJournal of Clinical Microbiology
Volume47
Issue number4
DOIs
StatePublished - Apr 1 2009

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Microbiota
Ecosystem
Gene Library
rRNA Genes
Cervix Uteri
Clone Cells
Health
Population

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Kim, T. K., Thomas, S. M., Ho, M., Sharma, S., Reich, C. I., Frank, J. A., ... Wilson, B. A. (2009). Heterogeneity of vaginal microbial communities within individuals. Journal of Clinical Microbiology, 47(4), 1181-1189. https://doi.org/10.1128/JCM.00854-08

Heterogeneity of vaginal microbial communities within individuals. / Kim, Tae Kyung; Thomas, Susan M.; Ho, Mengfei; Sharma, Shobha; Reich, Claudia I.; Frank, Jeremy A.; Yeater, Kathleen M.; Biggs, Diana R.; Nakamura, Noriko; Stumpf, Rebecca; Leigh, Steven R.; Tapping, Richard I.; Blanke, Steven R.; Slauch, James M.; Gaskins, H. Rex; Weisbaum, Jon S.; Olsen, Gary J.; Hoyer, Lois L.; Wilson, Brenda A.

In: Journal of Clinical Microbiology, Vol. 47, No. 4, 01.04.2009, p. 1181-1189.

Research output: Contribution to journalArticle

Kim, TK, Thomas, SM, Ho, M, Sharma, S, Reich, CI, Frank, JA, Yeater, KM, Biggs, DR, Nakamura, N, Stumpf, R, Leigh, SR, Tapping, RI, Blanke, SR, Slauch, JM, Gaskins, HR, Weisbaum, JS, Olsen, GJ, Hoyer, LL & Wilson, BA 2009, 'Heterogeneity of vaginal microbial communities within individuals', Journal of Clinical Microbiology, vol. 47, no. 4, pp. 1181-1189. https://doi.org/10.1128/JCM.00854-08
Kim TK, Thomas SM, Ho M, Sharma S, Reich CI, Frank JA et al. Heterogeneity of vaginal microbial communities within individuals. Journal of Clinical Microbiology. 2009 Apr 1;47(4):1181-1189. https://doi.org/10.1128/JCM.00854-08
Kim, Tae Kyung ; Thomas, Susan M. ; Ho, Mengfei ; Sharma, Shobha ; Reich, Claudia I. ; Frank, Jeremy A. ; Yeater, Kathleen M. ; Biggs, Diana R. ; Nakamura, Noriko ; Stumpf, Rebecca ; Leigh, Steven R. ; Tapping, Richard I. ; Blanke, Steven R. ; Slauch, James M. ; Gaskins, H. Rex ; Weisbaum, Jon S. ; Olsen, Gary J. ; Hoyer, Lois L. ; Wilson, Brenda A. / Heterogeneity of vaginal microbial communities within individuals. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 4. pp. 1181-1189.
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