Herring roe oil supplementation alters microglial cell gene expression and reduces peripheral inflammation after immune activation in a neonatal piglet model

Megan P. Caputo, Emily C. Radlowski, Marcus A. Lawson, Adrienne M. Antonson, Josephine E. Watson, Stephanie M. Matt, Brian J. Leyshon, Aditi Das, Rodney W Johnson

Research output: Contribution to journalArticle

Abstract

Neonatal brain development can be disrupted by infection that results in microglial cell activation and neuroinflammation. Studies indicate that polyunsaturated fatty acids (PUFAs) and their metabolites can resolve inflammation. It is not known if dietary PUFA increases lipid metabolites in brain or reduces neuroinflammation in neonates. We hypothesized that dietary PUFAs might suppress neuroinflammation by inhibiting pro-inflammatory cytokine over-production and promoting inflammatory resolution in the periphery and brain. Piglets were obtained on postnatal day (PD) 2 and randomly assigned to herring roe oil (HRO) or control (CON) diet. HRO was included at 2 g/kg powdered diet. HRO increased DHA levels in occipital lobe and the DHA to arachidonic acid (ARA) ratio in hippocampal tissue. HRO decreased ARA metabolites in occipital lobe. HRO failed to attenuate microglial pro-inflammatory cytokine production ex vivo. HRO did not affect fever or circulating resolvin D1 levels. HRO decreased circulating neutrophils and liver inflammatory gene expression, but increased resolution marker gene expression in liver post LPS. HRO upregulated CXCL16, TGFBR1, and C1QA in microglial cells. HRO supplementation exerted beneficial effects on inflammation in the periphery, but further studies are needed to evaluate the specific effects of omega-3 supplementation on microglial cell physiology in the neonate.

Original languageEnglish (US)
Pages (from-to)455-469
Number of pages15
JournalBrain, Behavior, and Immunity
Volume81
DOIs
StatePublished - Oct 2019

Fingerprint

Oils
Inflammation
Gene Expression
Unsaturated Fatty Acids
Occipital Lobe
Arachidonic Acid
Brain
Cytokines
Diet
Cell Physiological Phenomena
Liver
Neutrophils
Fever
Lipids
Infection

Keywords

  • Brain
  • Cytokine
  • Endocannabinoids
  • Gene expression
  • Herring roe oil
  • Inflammation
  • Lipopolysaccharide
  • Microglia
  • Piglet
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Herring roe oil supplementation alters microglial cell gene expression and reduces peripheral inflammation after immune activation in a neonatal piglet model. / Caputo, Megan P.; Radlowski, Emily C.; Lawson, Marcus A.; Antonson, Adrienne M.; Watson, Josephine E.; Matt, Stephanie M.; Leyshon, Brian J.; Das, Aditi; Johnson, Rodney W.

In: Brain, Behavior, and Immunity, Vol. 81, 10.2019, p. 455-469.

Research output: Contribution to journalArticle

Caputo, Megan P. ; Radlowski, Emily C. ; Lawson, Marcus A. ; Antonson, Adrienne M. ; Watson, Josephine E. ; Matt, Stephanie M. ; Leyshon, Brian J. ; Das, Aditi ; Johnson, Rodney W. / Herring roe oil supplementation alters microglial cell gene expression and reduces peripheral inflammation after immune activation in a neonatal piglet model. In: Brain, Behavior, and Immunity. 2019 ; Vol. 81. pp. 455-469.
@article{6e6651b805d8462097d4e8eae227b522,
title = "Herring roe oil supplementation alters microglial cell gene expression and reduces peripheral inflammation after immune activation in a neonatal piglet model",
abstract = "Neonatal brain development can be disrupted by infection that results in microglial cell activation and neuroinflammation. Studies indicate that polyunsaturated fatty acids (PUFAs) and their metabolites can resolve inflammation. It is not known if dietary PUFA increases lipid metabolites in brain or reduces neuroinflammation in neonates. We hypothesized that dietary PUFAs might suppress neuroinflammation by inhibiting pro-inflammatory cytokine over-production and promoting inflammatory resolution in the periphery and brain. Piglets were obtained on postnatal day (PD) 2 and randomly assigned to herring roe oil (HRO) or control (CON) diet. HRO was included at 2 g/kg powdered diet. HRO increased DHA levels in occipital lobe and the DHA to arachidonic acid (ARA) ratio in hippocampal tissue. HRO decreased ARA metabolites in occipital lobe. HRO failed to attenuate microglial pro-inflammatory cytokine production ex vivo. HRO did not affect fever or circulating resolvin D1 levels. HRO decreased circulating neutrophils and liver inflammatory gene expression, but increased resolution marker gene expression in liver post LPS. HRO upregulated CXCL16, TGFBR1, and C1QA in microglial cells. HRO supplementation exerted beneficial effects on inflammation in the periphery, but further studies are needed to evaluate the specific effects of omega-3 supplementation on microglial cell physiology in the neonate.",
keywords = "Brain, Cytokine, Endocannabinoids, Gene expression, Herring roe oil, Inflammation, Lipopolysaccharide, Microglia, Piglet, Polyunsaturated fatty acids",
author = "Caputo, {Megan P.} and Radlowski, {Emily C.} and Lawson, {Marcus A.} and Antonson, {Adrienne M.} and Watson, {Josephine E.} and Matt, {Stephanie M.} and Leyshon, {Brian J.} and Aditi Das and Johnson, {Rodney W}",
year = "2019",
month = "10",
doi = "10.1016/j.bbi.2019.06.046",
language = "English (US)",
volume = "81",
pages = "455--469",
journal = "Brain, Behavior, and Immunity",
issn = "0889-1591",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Herring roe oil supplementation alters microglial cell gene expression and reduces peripheral inflammation after immune activation in a neonatal piglet model

AU - Caputo, Megan P.

AU - Radlowski, Emily C.

AU - Lawson, Marcus A.

AU - Antonson, Adrienne M.

AU - Watson, Josephine E.

AU - Matt, Stephanie M.

AU - Leyshon, Brian J.

AU - Das, Aditi

AU - Johnson, Rodney W

PY - 2019/10

Y1 - 2019/10

N2 - Neonatal brain development can be disrupted by infection that results in microglial cell activation and neuroinflammation. Studies indicate that polyunsaturated fatty acids (PUFAs) and their metabolites can resolve inflammation. It is not known if dietary PUFA increases lipid metabolites in brain or reduces neuroinflammation in neonates. We hypothesized that dietary PUFAs might suppress neuroinflammation by inhibiting pro-inflammatory cytokine over-production and promoting inflammatory resolution in the periphery and brain. Piglets were obtained on postnatal day (PD) 2 and randomly assigned to herring roe oil (HRO) or control (CON) diet. HRO was included at 2 g/kg powdered diet. HRO increased DHA levels in occipital lobe and the DHA to arachidonic acid (ARA) ratio in hippocampal tissue. HRO decreased ARA metabolites in occipital lobe. HRO failed to attenuate microglial pro-inflammatory cytokine production ex vivo. HRO did not affect fever or circulating resolvin D1 levels. HRO decreased circulating neutrophils and liver inflammatory gene expression, but increased resolution marker gene expression in liver post LPS. HRO upregulated CXCL16, TGFBR1, and C1QA in microglial cells. HRO supplementation exerted beneficial effects on inflammation in the periphery, but further studies are needed to evaluate the specific effects of omega-3 supplementation on microglial cell physiology in the neonate.

AB - Neonatal brain development can be disrupted by infection that results in microglial cell activation and neuroinflammation. Studies indicate that polyunsaturated fatty acids (PUFAs) and their metabolites can resolve inflammation. It is not known if dietary PUFA increases lipid metabolites in brain or reduces neuroinflammation in neonates. We hypothesized that dietary PUFAs might suppress neuroinflammation by inhibiting pro-inflammatory cytokine over-production and promoting inflammatory resolution in the periphery and brain. Piglets were obtained on postnatal day (PD) 2 and randomly assigned to herring roe oil (HRO) or control (CON) diet. HRO was included at 2 g/kg powdered diet. HRO increased DHA levels in occipital lobe and the DHA to arachidonic acid (ARA) ratio in hippocampal tissue. HRO decreased ARA metabolites in occipital lobe. HRO failed to attenuate microglial pro-inflammatory cytokine production ex vivo. HRO did not affect fever or circulating resolvin D1 levels. HRO decreased circulating neutrophils and liver inflammatory gene expression, but increased resolution marker gene expression in liver post LPS. HRO upregulated CXCL16, TGFBR1, and C1QA in microglial cells. HRO supplementation exerted beneficial effects on inflammation in the periphery, but further studies are needed to evaluate the specific effects of omega-3 supplementation on microglial cell physiology in the neonate.

KW - Brain

KW - Cytokine

KW - Endocannabinoids

KW - Gene expression

KW - Herring roe oil

KW - Inflammation

KW - Lipopolysaccharide

KW - Microglia

KW - Piglet

KW - Polyunsaturated fatty acids

UR - http://www.scopus.com/inward/record.url?scp=85068574747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068574747&partnerID=8YFLogxK

U2 - 10.1016/j.bbi.2019.06.046

DO - 10.1016/j.bbi.2019.06.046

M3 - Article

AN - SCOPUS:85068574747

VL - 81

SP - 455

EP - 469

JO - Brain, Behavior, and Immunity

JF - Brain, Behavior, and Immunity

SN - 0889-1591

ER -