HER-2-mediated endocytosis of magnetic nanospheres and the implications in cell targeting and particle magnetization

Shy Chyi Wuang, Koon Gee Neoh, En Tang Kang, Daniel W. Pack, Deborah E Leckband

Research output: Contribution to journalArticle

Abstract

Polypyrrole-Fe3O4 nanospheres were synthesized via an emulsion polymerization method with hyaluronic acid as the surfactant. Hyaluronic acid offers the advantages of biocompatibility, cell adhesive property and the availability of functional groups for attachment of other molecules. The nanospheres were further functionalized with herceptin, and the efficacy of uptake of the functionalized nanospheres by human breast cancer cells was evaluated. It is envisioned that the combination of hyaluronic acid with its cell adhesive property and herceptin would result in high efficacy of internalization of the nanospheres by the cancer cells via a HER-2-mediated endocytosis. Our results showed that this is indeed the case and that the high concentration of herceptin-functionalized magnetic nanospheres in the cancer cells offers great potential in cancer cell targeting and treatment. In addition, the magnetic property of these nanospheres was also critically investigated and the magnetization was found to be affected by the particles' environment. The combination of these cell-targeting magnetic carriers with chemotherapeutic agents will be highly advantageous for the preferential killing of cancer cells in hyperthermia treatment.

Original languageEnglish (US)
Pages (from-to)2270-2279
Number of pages10
JournalBiomaterials
Volume29
Issue number14
DOIs
StatePublished - May 1 2008

Fingerprint

Nanospheres
Endocytosis
Magnetization
Hyaluronic acid
Cells
Hyaluronic Acid
Adhesives
Neoplasms
Emulsion polymerization
Polypyrroles
Biocompatibility
Surface-Active Agents
Functional groups
Magnetic properties
Surface active agents
Emulsions
Availability
Polymerization
Molecules
Fever

Keywords

  • Cell targeting
  • HER-2-mediated endocytosis
  • Herceptin
  • Magnetization
  • Polypyrrole-FeO nanospheres

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

HER-2-mediated endocytosis of magnetic nanospheres and the implications in cell targeting and particle magnetization. / Wuang, Shy Chyi; Neoh, Koon Gee; Kang, En Tang; Pack, Daniel W.; Leckband, Deborah E.

In: Biomaterials, Vol. 29, No. 14, 01.05.2008, p. 2270-2279.

Research output: Contribution to journalArticle

Wuang, Shy Chyi ; Neoh, Koon Gee ; Kang, En Tang ; Pack, Daniel W. ; Leckband, Deborah E. / HER-2-mediated endocytosis of magnetic nanospheres and the implications in cell targeting and particle magnetization. In: Biomaterials. 2008 ; Vol. 29, No. 14. pp. 2270-2279.
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