Hematology in an eastern massasauga (Sistrurus catenatus) population and the emergence of ophidiomyces in Illinois, USA

Matthew C Allender, Christopher A Phillips, Sarah J. Baker, Daniel B. Wylie, Amy Narotsky, Michael Joseph Dreslik

Research output: Contribution to journalArticle

Abstract

Disease events are threatening wildlife populations across North America. Specifically, mortality events due to Ophidiomyces (snake fungal disease; SFD) have been observed recently in snakes in Illinois, US. We investigated the health of a population of eastern massasaugas (Sistrurus catenatus) in south-central Illinois using 1) a meta-analysis of hematologic findings from 2004, 2011, 2013, and 2014; 2) a determination of the prevalence of SFD in snakes examined in 2013 and 2014; and 3) the examination of 184 museum specimens collected from 1999–2013 for signs and presence of SFD. For the meta-analysis and prevalence of SFD, hematologic analytes were reduced to three principle components that explained 67.5% of the cumulative variance. There were significant differences among one principle component (total white blood cell counts, monocytes, lymphocytes, and basophils) across years when it was highest in 2004 and 2014. The top general linear model explaining the difference in principle components included the main effects of year and stage, body condition index (BCI), and the interaction between stage and BCI. The prevalence of SFD was 18% (n=7) in 2013 and 24% (n=11) in 2014, and no hematologic analytes were associated with SFD. In museum specimens, Ophidiomyces DNA was first detected from an individual collected in 2000. Studies such as these, integrating multiple modalities of health, can elucidate the epidemiology of diseases that may pose conservation threats.

Original languageEnglish (US)
Pages (from-to)258-269
Number of pages12
JournalJournal of wildlife diseases
Volume52
Issue number2
DOIs
StatePublished - Apr 1 2016

Fingerprint

Sistrurus catenatus
hematology
snake
snakes
meta-analysis
body condition
museum
fungal disease
basophils
epidemiology
leukocyte count
monocytes
wildlife
lymphocytes
linear models
blood
DNA
mortality
health
index

Keywords

  • Eastern massasauga
  • Health
  • Hematology
  • Reptile
  • Sistrurus catenatus
  • Snake fungal disease

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Hematology in an eastern massasauga (Sistrurus catenatus) population and the emergence of ophidiomyces in Illinois, USA. / Allender, Matthew C; Phillips, Christopher A; Baker, Sarah J.; Wylie, Daniel B.; Narotsky, Amy; Dreslik, Michael Joseph.

In: Journal of wildlife diseases, Vol. 52, No. 2, 01.04.2016, p. 258-269.

Research output: Contribution to journalArticle

@article{e36da13347674f1c92bace241379b1f6,
title = "Hematology in an eastern massasauga (Sistrurus catenatus) population and the emergence of ophidiomyces in Illinois, USA",
abstract = "Disease events are threatening wildlife populations across North America. Specifically, mortality events due to Ophidiomyces (snake fungal disease; SFD) have been observed recently in snakes in Illinois, US. We investigated the health of a population of eastern massasaugas (Sistrurus catenatus) in south-central Illinois using 1) a meta-analysis of hematologic findings from 2004, 2011, 2013, and 2014; 2) a determination of the prevalence of SFD in snakes examined in 2013 and 2014; and 3) the examination of 184 museum specimens collected from 1999–2013 for signs and presence of SFD. For the meta-analysis and prevalence of SFD, hematologic analytes were reduced to three principle components that explained 67.5{\%} of the cumulative variance. There were significant differences among one principle component (total white blood cell counts, monocytes, lymphocytes, and basophils) across years when it was highest in 2004 and 2014. The top general linear model explaining the difference in principle components included the main effects of year and stage, body condition index (BCI), and the interaction between stage and BCI. The prevalence of SFD was 18{\%} (n=7) in 2013 and 24{\%} (n=11) in 2014, and no hematologic analytes were associated with SFD. In museum specimens, Ophidiomyces DNA was first detected from an individual collected in 2000. Studies such as these, integrating multiple modalities of health, can elucidate the epidemiology of diseases that may pose conservation threats.",
keywords = "Eastern massasauga, Health, Hematology, Reptile, Sistrurus catenatus, Snake fungal disease",
author = "Allender, {Matthew C} and Phillips, {Christopher A} and Baker, {Sarah J.} and Wylie, {Daniel B.} and Amy Narotsky and Dreslik, {Michael Joseph}",
year = "2016",
month = "4",
day = "1",
doi = "10.7589/2015-02-049",
language = "English (US)",
volume = "52",
pages = "258--269",
journal = "Journal of Wildlife Diseases",
issn = "0090-3558",
publisher = "Wildlife Disease Association, Inc.",
number = "2",

}

TY - JOUR

T1 - Hematology in an eastern massasauga (Sistrurus catenatus) population and the emergence of ophidiomyces in Illinois, USA

AU - Allender, Matthew C

AU - Phillips, Christopher A

AU - Baker, Sarah J.

AU - Wylie, Daniel B.

AU - Narotsky, Amy

AU - Dreslik, Michael Joseph

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Disease events are threatening wildlife populations across North America. Specifically, mortality events due to Ophidiomyces (snake fungal disease; SFD) have been observed recently in snakes in Illinois, US. We investigated the health of a population of eastern massasaugas (Sistrurus catenatus) in south-central Illinois using 1) a meta-analysis of hematologic findings from 2004, 2011, 2013, and 2014; 2) a determination of the prevalence of SFD in snakes examined in 2013 and 2014; and 3) the examination of 184 museum specimens collected from 1999–2013 for signs and presence of SFD. For the meta-analysis and prevalence of SFD, hematologic analytes were reduced to three principle components that explained 67.5% of the cumulative variance. There were significant differences among one principle component (total white blood cell counts, monocytes, lymphocytes, and basophils) across years when it was highest in 2004 and 2014. The top general linear model explaining the difference in principle components included the main effects of year and stage, body condition index (BCI), and the interaction between stage and BCI. The prevalence of SFD was 18% (n=7) in 2013 and 24% (n=11) in 2014, and no hematologic analytes were associated with SFD. In museum specimens, Ophidiomyces DNA was first detected from an individual collected in 2000. Studies such as these, integrating multiple modalities of health, can elucidate the epidemiology of diseases that may pose conservation threats.

AB - Disease events are threatening wildlife populations across North America. Specifically, mortality events due to Ophidiomyces (snake fungal disease; SFD) have been observed recently in snakes in Illinois, US. We investigated the health of a population of eastern massasaugas (Sistrurus catenatus) in south-central Illinois using 1) a meta-analysis of hematologic findings from 2004, 2011, 2013, and 2014; 2) a determination of the prevalence of SFD in snakes examined in 2013 and 2014; and 3) the examination of 184 museum specimens collected from 1999–2013 for signs and presence of SFD. For the meta-analysis and prevalence of SFD, hematologic analytes were reduced to three principle components that explained 67.5% of the cumulative variance. There were significant differences among one principle component (total white blood cell counts, monocytes, lymphocytes, and basophils) across years when it was highest in 2004 and 2014. The top general linear model explaining the difference in principle components included the main effects of year and stage, body condition index (BCI), and the interaction between stage and BCI. The prevalence of SFD was 18% (n=7) in 2013 and 24% (n=11) in 2014, and no hematologic analytes were associated with SFD. In museum specimens, Ophidiomyces DNA was first detected from an individual collected in 2000. Studies such as these, integrating multiple modalities of health, can elucidate the epidemiology of diseases that may pose conservation threats.

KW - Eastern massasauga

KW - Health

KW - Hematology

KW - Reptile

KW - Sistrurus catenatus

KW - Snake fungal disease

UR - http://www.scopus.com/inward/record.url?scp=84964766148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964766148&partnerID=8YFLogxK

U2 - 10.7589/2015-02-049

DO - 10.7589/2015-02-049

M3 - Article

VL - 52

SP - 258

EP - 269

JO - Journal of Wildlife Diseases

JF - Journal of Wildlife Diseases

SN - 0090-3558

IS - 2

ER -