TY - JOUR
T1 - Harvesting the genomic promise
T2 - Recombineering sequences for phenotypes
AU - Rogatcheva, Margarita B.
AU - Rund, Laurie A.
AU - Beever, Jonathan E.
AU - Schook, Lawrence B.
N1 - Funding Information:
This work was partially supported by grants from the USDA—National Research Initiative (2002-35205-12712), the USDA Cooperative State Research Service (AG2002-34480-11828), and the USDA Agricultural Research Service (Agreement No. 58-5438-2-313). The authors wish to recognize the contributions and assistance of C. Counter, Duke University Medical Center and E. Forsberg, Infigen. The authors also acknowledge the support and assistance of N. Copeland (NCI).
PY - 2003/11
Y1 - 2003/11
N2 - The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize genes contributing towards phenotypic variation. Whole-genome BAC contigs are also being constructed that will serve as the tiling path for genomic sequencing. Harvesting this genetic information for biological gain requires either genetic selection or the production of genetically modified animals. This later approach when coupled with nuclear transfer technology (NT) provides "clones" of genetically modified animals. However, to date, the production of genetically modified animals has been limited to either microinjection of small gene constructs into embryos with random insertion or complex gene constructs designed to knock-out targeted gene expression. Neither of these approaches provides for introducing directed genetic manipulation allowing for allelic substitution [knock-in], subsequent analyses of gene expression, and cloning. An alternative approach utilizing genomic sequence information and recombineering to direct gene targeting of specific porcine BACs is presented here.
AB - The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize genes contributing towards phenotypic variation. Whole-genome BAC contigs are also being constructed that will serve as the tiling path for genomic sequencing. Harvesting this genetic information for biological gain requires either genetic selection or the production of genetically modified animals. This later approach when coupled with nuclear transfer technology (NT) provides "clones" of genetically modified animals. However, to date, the production of genetically modified animals has been limited to either microinjection of small gene constructs into embryos with random insertion or complex gene constructs designed to knock-out targeted gene expression. Neither of these approaches provides for introducing directed genetic manipulation allowing for allelic substitution [knock-in], subsequent analyses of gene expression, and cloning. An alternative approach utilizing genomic sequence information and recombineering to direct gene targeting of specific porcine BACs is presented here.
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U2 - 10.1081/ABIO-120026481
DO - 10.1081/ABIO-120026481
M3 - Article
C2 - 14703070
AN - SCOPUS:0346255324
SN - 1049-5398
VL - 14
SP - 103
EP - 118
JO - Animal Biotechnology
JF - Animal Biotechnology
IS - 2
ER -